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Related Concept Videos

Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

591
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
591
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

766
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
766

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Updated: Oct 15, 2025

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
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Evaluation of standardized performance test methods for biomedical Raman spectroscopy.

Andrew M Fales1, Ilko K Ilev1, T Joshua Pfefer1

  • 1U.S. Food and Drug Administration, Center for Devices and Radiological Health, Silver Spring, Maryla, United States.

Journal of Biomedical Optics
|October 29, 2021
PubMed
Summary

Standardized testing is crucial for clinical Raman spectroscopy. A novel phantom demonstrated that device specifications alone do not predict in vivo performance, emphasizing the need for standardized phantom-based evaluation methods.

Keywords:
Raman spectroscopystandardstest methodsturbid phantom

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Area of Science:

  • Biomedical Optics
  • Spectroscopy
  • Medical Diagnostics

Background:

  • Raman spectroscopy offers molecular insights into diseases like cancer.
  • Clinical translation of Raman devices is hindered by a lack of standardized calibration and performance assessment methods for biological tissue analysis.

Purpose of the Study:

  • To advance the clinical translation of Raman-based devices.
  • To foster consensus on best practices for Raman system performance testing.

Main Methods:

  • Literature and standards review for common bench testing methods (intensity correction, wavenumber calibration, noise, resolution, sensitivity).
  • Development and use of a novel 3D-printed turbid phantom to assess depth sensitivity.
  • Implementation of these methods on three distinct fiber-optic-probe-based Raman systems.

Main Results:

  • Traditional testing methods revealed system-specific differences (detectors, spectrometers, data processing).
  • The turbid phantom highlighted the significant influence of illumination-collection geometry on measurement quality.

Conclusions:

  • Device specifications are insufficient predictors of in vivo performance.
  • Standardized, phantom-based testing methodologies are essential for reliable evaluation of Raman devices for biomedical applications.