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Updated: Oct 15, 2025
Exosomal miRNA Analysis in Non-small Cell Lung Cancer NSCLC Patients' Plasma Through qPCR: A Feasible Liquid Biopsy Tool
Published on: May 27, 2016
Construction of ceRNA network to identify the lncRNA and mRNA related to non-small cell lung cancer
Kui Xiao1,2,3, Yang Wang4, Lihua Zhou5
1Department of Respiratory and Critical Care Medicine, The Second Xiangya Hospital of Central South University, Changsha, Hunan, China.
Background:
Non-small cell lung cancer (NSCLC) harms human health, but its pathogenesis remains unclear. We wish to provide more molecular therapeutic targets for NSCLC.
Methods:
The NSCLC tissue and normal tissue samples were screened for genetic comparison in the TCGA database. The predicted lncRNA and mRNA in BEAS2B and A549 cells were detected.
Results:
Volcano plot displayed differentially expressed lncRNAs and mRNAs in adjacent tissues and NSCLC tissues. The survival curve showed that the lncRNA and mRNA had a significant impact on the patient's survival. The results of GO term enrichment analysis indicated that mRNA functions were enriched in cell cycle-related pathways. In the ceRNA interaction network, 13 lncRNAs and 20 miRNAs were found to have an interactive relationship. Finally, 3 significantly different lncRNAs (LINC00968, lnc-FAM92A-9 and lnc-PTGFR-1) and 6 mRNAs (CTCFL, KRT5, LY6D, TMEM, GBP6, and TMEM179) with potential therapeutic significance were screened out. And the cell experiment verified our results.
Conclusion:
We screened out clinically significant 3 lncRNAs and 6 mRNAs involved in the ceRNA network, which were the key to our future research on the treatment of NSCLC.
Insights
Researchers identified 3 lncRNAs and 6 mRNAs involved in the ceRNA network as potential therapeutic targets for non-small cell lung cancer (NSCLC). This discovery advances understanding of NSCLC pathogenesis and treatment strategies.
Area of Science:
- Oncology
- Molecular Biology
- Bioinformatics
Background:
- Non-small cell lung cancer (NSCLC) poses a significant health burden, with its underlying pathogenesis not fully understood.
- Identifying novel molecular targets is crucial for developing effective NSCLC therapies.
Purpose of the Study:
- To identify potential molecular therapeutic targets for non-small cell lung cancer (NSCLC).
- To elucidate the roles of lncRNAs and mRNAs in NSCLC development through ceRNA network analysis.
Main Methods:
- Utilized TCGA database for genetic comparison of NSCLC and adjacent normal tissues.
- Analyzed differentially expressed lncRNAs and mRNAs using bioinformatics tools.
- Constructed a competing endogenous RNA (ceRNA) interaction network.
- Validated findings through cell experiments.
Main Results:
- Identified significant differences in lncRNA and mRNA expression between NSCLC and normal tissues.
- Established a ceRNA network involving 13 lncRNAs and 20 miRNAs.
- Screened 3 differentially expressed lncRNAs (LINC00968, lnc-FAM92A-9, lnc-PTGFR-1) and 6 mRNAs (CTCFL, KRT5, LY6D, TMEM, GBP6, TMEM179) with therapeutic potential.
- Demonstrated the impact of these molecules on patient survival and cell cycle pathways.
Conclusions:
- Successfully screened 3 lncRNAs and 6 mRNAs critical for NSCLC pathogenesis within the ceRNA network.
- These identified molecules represent key targets for future NSCLC treatment research.
