Related Experiment Video
Updated: Oct 15, 2025
06:24
Oncogene Expression Analysis with Alterations in pH in a Pancreatic Ductal Cell Line
Published on: April 11, 2025
609
Expression of Oncogenic Molecules in Pediatric Ulcerative Colitis
Nobuyasu Arai1, Takahiro Kudo2, Kazuhide Tokita2
1Department of Pediatric and Adolescent Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan, n-arai@juntendo.ac.jp.
Digestion
|October 31, 2021
Summary
Pediatric ulcerative colitis (UC) patients show increased expression of cancer-related genes, PIM2 and SPI1. This suggests chronic inflammation in childhood UC may elevate colorectal cancer risk.
Area of Science:
- Gastroenterology
- Pediatric Oncology
- Molecular Biology
Background:
- Long-term ulcerative colitis (UC) increases colorectal cancer risk in adults.
- This association lacks genetic analysis in pediatric UC.
- Investigating cancer-related gene expression in pediatric UC is crucial.
Purpose of the Study:
- To examine cancer-related gene expression in pediatric UC patients.
- To assess the association between gene expression and colorectal cancer risk in children with UC.
Main Methods:
- Microarray analysis of rectal mucosa biopsy specimens from pediatric UC patients (active and remission phases) and controls.
- Validation using real-time polymerase chain reaction (PCR) and immunohistochemical staining.
- Analysis of cancer-related genes including PIM2, SPI1, TP53, and APC.
Main Results:
- Microarray identified higher PIM2 and SPI1 expression in active UC.
- Real-time PCR confirmed elevated PIM2 and SPI1, and reduced TP53 and APC in active UC.
- Immunohistochemistry supported PCR findings for all analyzed genes.
Conclusions:
- Pediatric UC patients exhibit significantly altered expression of cancer-related genes compared to controls.
- Gastrointestinal inflammation in childhood-onset UC elevates cancer-related gene expression.
- Chronic inflammation in pediatric UC may increase future colorectal cancer risk.
Related Concept Videos
01:27Inflammatory Bowel Disease I: Ulcerative Colitis
413
Introduction
Inflammatory bowel disease, or IBD, encompasses a group of disorders characterized by chronic inflammation or ulceration of the gastrointestinal tract.
Risk Factors
The exact cause of IBD remains unclear, although it is believed to be due to a mix of genetic, environmental, microbial, and immune factors. Genetic factors are significant in determining susceptibility to IBD, with family history being a critical risk factor. Individuals with a first-degree relative who has IBD are at...
Inflammatory bowel disease, or IBD, encompasses a group of disorders characterized by chronic inflammation or ulceration of the gastrointestinal tract.
Risk Factors
The exact cause of IBD remains unclear, although it is believed to be due to a mix of genetic, environmental, microbial, and immune factors. Genetic factors are significant in determining susceptibility to IBD, with family history being a critical risk factor. Individuals with a first-degree relative who has IBD are at...
413
01:33Cancer-Critical Genes I: Proto-oncogenes
9.3K
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
9.3K
01:06Induced Pluripotent Stem Cells
4.5K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
Somatic...
4.5K
01:30Inflammatory Bowel Disease II: Crohn's Disease
451
Introduction
Inflammatory bowel disease, commonly known as IBD, refers to a collection of disorders that lead to persistent inflammation of the gastrointestinal tract. The two types of IBD are ulcerative colitis, which impacts the colon, and Crohn's disease, which can involve any part of the gastrointestinal segment.
Crohn's disease
Crohn's disease is a chronic, systemic inflammatory bowel disease (IBD) that predominantly affects the gastrointestinal tract. It is marked by...
Inflammatory bowel disease, commonly known as IBD, refers to a collection of disorders that lead to persistent inflammation of the gastrointestinal tract. The two types of IBD are ulcerative colitis, which impacts the colon, and Crohn's disease, which can involve any part of the gastrointestinal segment.
Crohn's disease
Crohn's disease is a chronic, systemic inflammatory bowel disease (IBD) that predominantly affects the gastrointestinal tract. It is marked by...
451
02:23Abnormal Proliferation
4.7K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.7K
02:07Tumor Progression
6.6K
Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
6.6K