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RNAPhaSep: a resource of RNAs undergoing phase separation
Haibo Zhu1,2, Hao Fu1,2, Tianyu Cui3
1Department of Intelligent Medical Engineering, School of Medical Technology and Engineering, Fujian Medical University, Fuzhou 350122, China.
Nucleic Acids Research
|October 31, 2021
Summary
This study introduces RNAPhaSep, a database detailing RNA liquid-liquid phase separation (LLPS). It highlights RNA
Area of Science:
- Biochemistry and Molecular Biology
- Cell Biology
- Genomics
Background:
- Liquid-liquid phase separation (LLPS) is crucial for cellular organization, forming membraneless organelles.
- Research has primarily focused on protein involvement in LLPS, particularly RNA-binding proteins.
- Emerging evidence shows RNAs can act as scaffolds, initiating or nucleating phase separation events.
Purpose of the Study:
- To consolidate and provide access to dispersed knowledge on RNA-mediated LLPS.
- To introduce RNAPhaSep, a manually curated database of RNAs involved in LLPS.
- To facilitate research into the relationship between RNA properties and phase behavior.
Main Methods:
- Manual curation of published literature on RNA phase separation.
- Inclusion of experimentally validated RNA self-assembly and RNA-protein co-phase separation events.
- Integration of RNA, protein, and experimental data with annotations from multiple databases.
Main Results:
- The RNAPhaSep database contains 1113 curated entries.
- Entries detail RNA information, protein partners, and phase separation experimental conditions.
- Data includes validated RNA self-assembly and RNA-protein co-involved phase separation.
Conclusions:
- RNAPhaSep serves as a valuable resource for studying RNA's role in LLPS.
- The database can enhance understanding of LLPS in cellular functions and diseases.
- It supports exploration of the link between RNA characteristics and phase separation phenomena.
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