The effect of 4-hexylresorinol administration on NAD+ level and SIRT activity in Saos-2 cells
In-Song Lee1, Jun-Ho Chang1, Dae-Won Kim2
1Department of Orthodontics, School of Dentistry, Seoul National University, Seoul, 3080, Republic of Korea.
Background:
4-hexylresorcinol (4HR) has been shown to have anti-oxidant activity similar to that of resveratrol. As resveratrol increases sirtuin (SIRT) activity, 4HR might behave similarly to resveratrol.
Method:
In this study, the expression levels of SIRT1, SIRT3, and SIRT6 were evaluated after 4HR administration (1-100 μM). As NAD+ is a substrate for SIRTs, its levels with SIRT activity were also studied.
Results:
In the results, SIRT3 (100 μM at 24 h) and SIRT6 (1-100 μM at 24 h and 10 μM at 8 h) were shown to have significantly higher expression levels compared to untreated control (p < 0.05). Pan-SIRT activity and the NAD+ level was significantly increased compared to that of the untreated control (p < 0.05; 10 and 100 μM at 24 h).
Conclusion:
4HR administration increased SIRT activity and the NAD+ level in Saos-2 cells.
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