Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Editing02:23

RNA Editing

9.3K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.3K
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

7.8K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
7.8K
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

7.4K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
7.4K
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

5.9K
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
5.9K
Regulated mRNA Transport02:22

Regulated mRNA Transport

6.5K
In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing...
6.5K
Pre-mRNA Processing: Modification of pre-mRNA Ends01:35

Pre-mRNA Processing: Modification of pre-mRNA Ends

10.9K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
10.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

PAPOLA-mediated hyperactive polyadenylation promotes leukemogenesis and leukemia stem cell self-renewal through metabolic reprogramming.

Nature cancer·2026
Same author

Antibody reveals conformational latch regulating dimerization in β- and γ-herpesvirus proteases.

Nature communications·2026
Same author

CryoROLE: describing large inter-domain rotation in single particle cryo-EM.

bioRxiv : the preprint server for biology·2026
Same author

Light-regulated reconfigurable MXene-hydrogel gas sensing system via machine learning.

Journal of colloid and interface science·2026
Same author

Association Between Immunotherapy and Generalization in Ocular Myasthenia Gravis as a Function of Antibody Status.

Revista de neurologia·2026
Same author

Mechanisms of yield and quality enhancement in <i>Panax Vietnamensis</i>: A comparison of three artificial cultivation models.

Journal of ginseng research·2026

Related Experiment Video

Updated: Oct 14, 2025

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis
08:50

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

Published on: May 14, 2020

6.9K

METTL3-Mediated m6A RNA Modification Regulates Corneal Injury Repair.

Yarong Dai1, Maosheng Cheng2,3, Siyan Zhang1

  • 1Institute for Advanced Study, Shenzhen University, 518067, China.

Stem Cells International
|November 1, 2021
PubMed
Summary

Removing METTL3 in limbal stem cells enhances corneal injury repair by promoting cell proliferation and migration. This study reveals the crucial role of N6-methyladenosine (m6A) RNA modification in healing eye injuries.

More Related Videos

Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry
08:45

Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry

Published on: April 21, 2022

2.5K
Corneal Epithelial Abrasion with Ocular Burr As a Model for Cornea Wound Healing
07:28

Corneal Epithelial Abrasion with Ocular Burr As a Model for Cornea Wound Healing

Published on: July 10, 2018

20.7K

Related Experiment Videos

Last Updated: Oct 14, 2025

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis
08:50

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

Published on: May 14, 2020

6.9K
Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry
08:45

Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry

Published on: April 21, 2022

2.5K
Corneal Epithelial Abrasion with Ocular Burr As a Model for Cornea Wound Healing
07:28

Corneal Epithelial Abrasion with Ocular Burr As a Model for Cornea Wound Healing

Published on: July 10, 2018

20.7K

Area of Science:

  • Biochemistry
  • Stem Cell Biology
  • Ophthalmology

Background:

  • Limbal stem cells are vital for corneal regeneration and repair.
  • N6-methyladenosine (m6A) mRNA modifications regulate stem cell functions.
  • The role of m6A in corneal injury repair is currently unknown.

Purpose of the Study:

  • To investigate the function of m6A RNA modification in corneal injury repair.
  • To elucidate the role of METTL3 in limbal stem cells during alkali burn injury.
  • To identify m6A targets involved in corneal repair.

Main Methods:

  • Generation of a limbal stem cell-specific METTL3 knockout mouse model.
  • Induction of corneal alkali burn injury.
  • Assessment of cell proliferation and migration in vivo.
  • m6A modification profiling.

Main Results:

  • METTL3 knockout in limbal stem cells significantly accelerated corneal injury repair.
  • Enhanced in vivo cell proliferation and migration were observed in knockout mice.
  • AHNAK and DDIT4 were identified as novel m6A targets regulating stem cell function.
  • m6A modification is essential for effective corneal wound healing.

Conclusions:

  • METTL3-mediated m6A modification plays a critical role in regulating corneal injury repair.
  • Targeting m6A pathways offers potential therapeutic strategies for corneal diseases.
  • This research provides new insights into stem cell-based corneal regeneration.