Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Determinants of natural killer cell-mediated antibody dependent cellular cytotoxicity in SARS-CoV-2 antibodies.

PLoS pathogens·2026

Same author

Adverse maternal and fetal outcomes in mouse models of prenatal infections.

Wellcome open research·2025

Same author

Innate Lymphoid Cells in Reproductive Health and Disease.

European journal of immunology·2025

Same author

Mamma MIA! Decidual NK cells involved in fetal neurodevelopment.

Immunity·2025

Same author

Kir6.1, a component of an ATP-sensitive potassium channel, regulates natural killer cell development.

Frontiers in immunology·2024

Same author

Do K<sub>ATP</sub> channels have a role in immunity?

Frontiers in immunology·2024

Search research articles

Related Experiment Video

Updated: Oct 14, 2025

Isolation of Uterine Innate Lymphoid Cells for Analysis by Flow Cytometry

Isolation of Uterine Innate Lymphoid Cells for Analysis by Flow Cytometry

Published on: October 14, 2021

Isolation of Uterine Innate Lymphoid Cells for Analysis by Flow Cytometry.

Delphine M Depierreux¹, Emily Seshadri¹, Evgeniya V Shmeleva¹

¹Department of Obstetrics and Gynaecology, National Institute for Health Research Cambridge Biomedical Research Centre, University of Cambridge School of Clinical Medicine; Centre for Trophoblast Research, University of Cambridge.

Journal of Visualized Experiments : Jove

|November 1, 2021

Summary

This study presents a straightforward flow cytometry method for isolating and analyzing group 1 uterine innate lymphoid cells (g1 uILCs) in pregnant mice. This technique enables detailed investigation into g1 uILC characteristics and their role during pregnancy.

More Related Videos

Isolation and Flow Cytometric Characterization of Murine Small Intestinal Lymphocytes

Isolation and Flow Cytometric Characterization of Murine Small Intestinal Lymphocytes

Published on: May 8, 2016

Isolating Lymphocytes from the Mouse Small Intestinal Immune System

Isolating Lymphocytes from the Mouse Small Intestinal Immune System

Published on: February 28, 2018

Related Experiment Videos

Last Updated: Oct 14, 2025

Isolation of Uterine Innate Lymphoid Cells for Analysis by Flow Cytometry

Isolation of Uterine Innate Lymphoid Cells for Analysis by Flow Cytometry

Published on: October 14, 2021

Isolation and Flow Cytometric Characterization of Murine Small Intestinal Lymphocytes

Isolation and Flow Cytometric Characterization of Murine Small Intestinal Lymphocytes

Published on: May 8, 2016

Isolating Lymphocytes from the Mouse Small Intestinal Immune System

Isolating Lymphocytes from the Mouse Small Intestinal Immune System

Published on: February 28, 2018

Area of Science:

Immunology
Reproductive Biology
Cell Biology

Background:

Innate lymphoid cells (ILCs) play crucial roles in tissue homeostasis and immunity.
Group 1 uterine innate lymphoid cells (g1 uILCs) are critical immune cells in the pregnant uterus.
Characterizing g1 uILCs requires efficient isolation and phenotyping methods.

Purpose of the Study:

To describe a simple and effective method for isolating and phenotyping mouse g1 uILCs from pregnant uteri.
To enable the study of g1 uILC heterogeneity, function, and response to pregnancy-related factors.

Main Methods:

Time mating of mice to synchronize pregnant dams.
Mechanical and enzymatic digestion of pregnant uteri to create single-cell suspensions.
Flow cytometry and FACS (Fluorescence-activated cell sorting) for g1 uILC identification and phenotyping.

Main Results:

A reliable protocol for isolating and phenotyping g1 uILCs from individual pregnant mouse uteri was established.
The method allows for the analysis of g1 uILC heterogeneity and gene expression.
The protocol has been successfully used to study g1 uILC responses to maternal and fetal factors during pregnancy.

Conclusions:

This method provides a valuable tool for researchers studying uterine immunity during pregnancy.
It facilitates a deeper understanding of g1 uILC roles in reproductive health and pregnancy outcomes.
Despite loss of spatial information, the protocol is effective for functional and molecular analyses of g1 uILCs.