Related Experiment Video
Updated: Oct 14, 2025

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Rapid Protocol for Preparation of Electrocompetent Escherichia coli and Vibrio cholerae
Published on: October 8, 2013
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One-Step Preparation of Competent E. coli: Transformation and Storage of Bacterial Cells in the Same Solution
Cold Spring Harbor Protocols
|November 2, 2021
Abstract:
This protocol describes a convenient method for the preparation, use, and storage of competent Escherichia coli The reported transformation efficiency of this method is ∼5 × 107 transformants/µg of plasmid DNA.

