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One-Step Preparation of Competent E. coli: Transformation and Storage of Bacterial Cells in the Same Solution

Michael R Green, Joseph Sambrook

    Cold Spring Harbor Protocols
    |November 2, 2021
    PubMed

    Abstract:

    This protocol describes a convenient method for the preparation, use, and storage of competent Escherichia coli The reported transformation efficiency of this method is ∼5 × 107 transformants/µg of plasmid DNA.

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    Microbial communities are dynamic environments where cell lysis releases free DNA into the surroundings. Other cells can take up this extracellular DNA through a process known as transformation.When a cell incorporates this foreign DNA into its genome, resulting in genetic modification, the process is known as transformation. Cells capable of this process are termed competent. Competence can be natural, as observed in certain bacteria and archaea, or artificially induced in the...
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