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Updated: Oct 14, 2025

Visualization of Pseudomonas aeruginosa within the Sputum of Cystic Fibrosis Patients
07:25

Visualization of Pseudomonas aeruginosa within the Sputum of Cystic Fibrosis Patients

Published on: July 16, 2020

3.3K

MICROBIOLOGICAL FEATURES OF STAPHYLOCOCCUS AUREUS ISOLATED FROM RESPIRATORY TRACT OF CHILDREN WITH CYSTIC FIBROSIS

Oksana Ishchenko1, Iryna Koshova1, Inna Borysova1

  • 1DNIPRO STATE MEDICAL UNIVERSITY, DNIPRO, UKRAINE.

Wiadomosci Lekarskie (Warsaw, Poland : 1960)
|November 2, 2021
PubMed

Insights

Staphylococcus aureus infection is common in children with Cystic Fibrosis, with small colony variants appearing frequently. While isolates show good antimicrobial sensitivity, increasing resistance in modified phenotypes is a concern.

Area of Science:

  • Medical Microbiology
  • Infectious Diseases
  • Pediatrics

Background:

  • Staphylococcus aureus is a significant pathogen in Cystic Fibrosis (CF) patients.
  • Understanding its prevalence and antimicrobial susceptibility in pediatric CF populations is crucial for effective treatment.

Purpose of the Study:

  • To determine the prevalence of Staphylococcus aureus in children with CF in the Dnieper region.
  • To characterize the microbiological features of isolates.
  • To assess their susceptibility to various antimicrobial agents.

Main Methods:

  • Bacteriological analysis of sputum, tracheobronchial lavage, and pharyngeal swabs from genetically confirmed CF children.
  • Screening for Staphylococcus aureus small colony variants (SCVs) after 48-hour incubation.
  • Antimicrobial susceptibility testing using the disk-diffusion method (EUCAST 2019).

Main Results:

  • Staphylococcus aureus was identified in 40.8% of the 21 enrolled CF children.
  • Small colony variants (SCVs) of Staphylococcus aureus were detected in 21.6% of cases.
  • Higher association of Staphylococcus aureus auxotrophs with Pseudomonas aeruginosa was observed. 3rd-generation aminoglycosides, fluoroquinolones, linezolid, rifampicin, and tetracyclines showed good activity, but increased resistance to cefoxitin and gentamicin was noted in auxotrophs.

Conclusions:

  • Staphylococcus aureus infection is prevalent in pediatric CF patients.
  • The emergence of auxotrophs is linked to aminoglycoside/co-trimoxazole treatment and Pseudomonas aeruginosa co-infection.
  • While isolates generally remain sensitive, a concerning trend of increasing resistance in auxotroph-modified phenotypes requires monitoring.
Abstract

Keywords:
Staphylococcus aureuschronic respiratory tract infectioncystic fibrosissmall colony variants

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