Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Photochemical Electrocyclic Reactions: Stereochemistry01:26

Photochemical Electrocyclic Reactions: Stereochemistry

1.9K
The absorption of UV–visible light by conjugated systems causes the promotion of an electron from the ground state to the excited state. Consequently, photochemical electrocyclic reactions proceed via the excited-state HOMO rather than the ground-state HOMO. Since the ground- and excited-state HOMOs have different symmetries, the stereochemical outcome of electrocyclic reactions depends on the mode of activation; i.e., thermal or photochemical.
Selection Rules: Photochemical Activation
1.9K
Deactivation Processes: Jablonski Diagram01:25

Deactivation Processes: Jablonski Diagram

1.0K
Luminescence, the emission of light by a substance that has absorbed energy, is a process that involves the interaction of molecules with light. The energy-level diagram, or Jablonski diagram, is a graphical representation of these interactions, illustrating the various states and transitions a molecule can undergo. In a typical Jablonski diagram, the lowest horizontal line represents the ground-state energy of the molecule, which is usually a singlet state. This state represents the energies...
1.0K
Nuclear Overhauser Enhancement (NOE)01:07

Nuclear Overhauser Enhancement (NOE)

906
Irradiation of a spin-active nucleus causes an increase or decrease in the signal intensity of neighboring nuclei that are not necessarily chemically bonded or involved in J-coupling.  This phenomenon, called the Nuclear Overhauser Enhancement (NOE), results from through-space interactions between the nuclear spins. The NOE effect decreases with increasing internuclear distance and is generally not observed beyond 4 angstroms. In NOE, dipole-dipole interactions between neighboring...
906
Atomic Nuclei: Nuclear Relaxation Processes01:23

Atomic Nuclei: Nuclear Relaxation Processes

765
In the absence of an external magnetic field, nuclear spin states are degenerate and randomly oriented. When a magnetic field is applied, the spins begin to precess and orient themselves along (lower energy) or against (higher energy) the direction of the field. At equilibrium, a slight excess population of spins exists in the lower energy state. Because the direction of the magnetic field is fixed as the z-axis,  the precessing magnetic moments are randomly oriented around the z-axis.
765
Biasing of P-N Junction01:16

Biasing of P-N Junction

1.1K
The operation of a p-n junction diode involves various biasing conditions, including forward bias, reverse bias, and equilibrium.
In equilibrium, no external voltage is applied across the p-n junction. The depletion region is formed at the junction interface due to the diffusion of carriers, which leaves behind charged dopants, acceptors on the p-side, and donors on the n-side. These immobile charges create an electric field that prevents further diffusion of carriers. The related energy band...
1.1K
Generating Electromagnetic Radiations01:10

Generating Electromagnetic Radiations

4.8K
The German physicist Heinrich Hertz (1857–1894) was the first to generate and detect certain types of electromagnetic waves in the laboratory. Starting in 1887, he performed a series of experiments that confirmed the existence of electromagnetic waves and verified that they travel at the speed of light. Hertz used an alternating-current RLC (resistor-inductor-capacitor) circuit that resonated at a known frequency and connected it to a loop of wire. High voltages induced across the gap in...
4.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Voltage dynamics of cortical dendrites in vivo.

Nature neuroscience·2026
Same author

Correction to "Optical Single-Channel Recording via Diffusional Confinement in Membrane Tethers".

ACS nano·2026
Same author

A dendrite-resolved, <i>in vivo</i> transfer function from spike patterns to dendritic Ca<sup>2</sup>.

bioRxiv : the preprint server for biology·2026
Same author

Ether lipids influence cancer cell fate by modulating iron uptake.

Nature communications·2026
Same author

Fast dendritic excitations primarily mediate back-propagation in CA1 pyramidal neurons during behavior.

bioRxiv : the preprint server for biology·2026
Same author

Erratum: Enhancement of Rydberg Blockade via Microwave Dressing [Phys. Rev. Lett. 134, 123404 (2025)].

Physical review letters·2025
Same journal

Stability of Some Ternary 13-Atom Icosahedral Clusters Assessed with Geometric, Electronic, and Thermodynamic Criteria.

The journal of physical chemistry. A·2026
Same journal

A Three-Phase Distribution Method for Quantifying the Intermolecular Interactions.

The journal of physical chemistry. A·2026
Same journal

Cooperative Effects in the Inverse Coordination Complexes of Aromatic Azines and Tin(IV) Halides.

The journal of physical chemistry. A·2026
Same journal

The Infrared Spectra of Neutral Dimethyl-Sulfide, -Disulfide and -Sulfoxide Biomarkers in Molecular Beams.

The journal of physical chemistry. A·2026
Same journal

Photoinduced Charge-Transfer Suppresses Triplet Formation Efficiency in Thiocoumarins: Evidence from Ultrafast Spectroscopy and Theoretical Calculations.

The journal of physical chemistry. A·2026
Same journal

Porphyrin Aggregation Revisited: From the Four-Orbital Gouterman Model to an Eight-Orbital Framework in Porphin H-Dimers.

The journal of physical chemistry. A·2026
See all related articles

Related Experiment Video

Updated: Oct 14, 2025

Local Field Fluorescence Microscopy: Imaging Cellular Signals in Intact Hearts
10:33

Local Field Fluorescence Microscopy: Imaging Cellular Signals in Intact Hearts

Published on: March 8, 2017

8.4K

Which Way Does Stimulated Emission Go?

J David Wong-Campos1, J V Porto2, Adam E Cohen1,3

  • 1Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, United States.

The Journal of Physical Chemistry. A
|November 3, 2021
PubMed
Summary
This summary is machine-generated.

This study explores using stimulated emission to create images by analyzing light scattering from excited molecules. The findings reveal new optical imaging techniques based on fluorophore excited states.

More Related Videos

Direct Imaging of Laser-driven Ultrafast Molecular Rotation
10:52

Direct Imaging of Laser-driven Ultrafast Molecular Rotation

Published on: February 4, 2017

9.8K
Photostimulation by Femtosecond Laser Activates Extracellular-signal-regulated Kinase ERK Signaling or Mitochondrial Events in Target Cells
11:00

Photostimulation by Femtosecond Laser Activates Extracellular-signal-regulated Kinase ERK Signaling or Mitochondrial Events in Target Cells

Published on: July 6, 2019

6.9K

Related Experiment Videos

Last Updated: Oct 14, 2025

Local Field Fluorescence Microscopy: Imaging Cellular Signals in Intact Hearts
10:33

Local Field Fluorescence Microscopy: Imaging Cellular Signals in Intact Hearts

Published on: March 8, 2017

8.4K
Direct Imaging of Laser-driven Ultrafast Molecular Rotation
10:52

Direct Imaging of Laser-driven Ultrafast Molecular Rotation

Published on: February 4, 2017

9.8K
Photostimulation by Femtosecond Laser Activates Extracellular-signal-regulated Kinase ERK Signaling or Mitochondrial Events in Target Cells
11:00

Photostimulation by Femtosecond Laser Activates Extracellular-signal-regulated Kinase ERK Signaling or Mitochondrial Events in Target Cells

Published on: July 6, 2019

6.9K

Area of Science:

  • Optics and Photonics
  • Biophysics

Background:

  • Stimulated emission is a fundamental process in lasers and fluorescence.
  • Optical imaging typically relies on absorption or spontaneous emission.
  • The interplay between stimulated emission and light scattering is not fully exploited for imaging.

Purpose of the Study:

  • To investigate the potential of using stimulated emission to form images.
  • To analyze light scattering from excited chromophores.
  • To explore novel optical imaging modalities.

Main Methods:

  • Studying light scatter from an assembly of excited chromophores.
  • Applying principles of the Optical Theorem.
  • Analyzing dependencies on scattering direction, wavelength, and chromophore configuration.

Main Results:

  • Stimulated emission is intrinsically linked to excited state Rayleigh scattering (Optical Theorem).
  • Both stimulated emission and Rayleigh scattering can be utilized for image formation.
  • Image formation capabilities differ based on scattering properties and molecular arrangements.

Conclusions:

  • Stimulated emission can be harnessed for optical imaging.
  • Excited state Rayleigh scattering provides an alternative pathway for imaging.
  • New imaging approaches utilizing fluorophore excited states are proposed.