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Identification of key genes involved in Brg1 mutation-induced cataract using bioinformatics analyses with publicly
Chen Li1, Jianqing Li1, Peirong Lu1
1Department of Ophthalmology, the First Affiliated Hospital of Soochow University, Shizi Street 188, Suzhou, 21006, Jiangsu Province, China.
Acta Biochimica Polonica
|November 3, 2021
Summary
A Brahma-related gene 1 (Brg1) mutation impacts mouse lens development, affecting cell cycle and transport genes. Key genes like HSP90ab1, Polr2e, Cdc20, and Polr2c may be therapeutic targets for cataract treatment.
Area of Science:
- Genetics
- Ophthalmology
- Molecular Biology
Background:
- Cataract is a prevalent age-related eye disease.
- The role of Brahma-related gene 1 (Brg1) in cataract formation is not fully understood.
- Investigating Brg1's mechanism in cataractogenesis is crucial for potential treatments.
Purpose of the Study:
- To investigate the effects of a Brg1 mutation on lens development in mice.
- To identify genes and pathways affected by Brg1 mutations in cataract formation.
- To explore potential therapeutic targets for cataract treatment based on Brg1's role.
Main Methods:
- Comparative analysis of mRNA profiles from Brg1 transgenic and wild-type mouse lenses using Gene Expression Omnibus (GEO) data.
- Identification of differentially expressed genes (DEGs) using NetworkAnalyst.
- Pathway analysis (GO, KEGG, Reactome) of DEGs via Metascape.
- Construction and analysis of protein-protein interaction (PPI) networks using STRING and Cytoscape.
Main Results:
- 323 differentially expressed genes (DEGs) were identified.
- Upregulated DEGs were enriched in cell cycle regulation and mRNA splicing.
- Downregulated DEGs were associated with organ hydroxy compound transport and neuron migration.
- Heat shock protein 90 alpha (HSP90ab1), polymerase (RNA) II subunits (Polr2e, Polr2c), and cell division cycle 20 (Cdc20) showed high connectivity in the PPI network.
Conclusions:
- Brg1 mutation alters the expression of critical genes including HSP90ab1, Polr2e, Cdc20, and Polr2c.
- These altered genes are implicated in the development of cataracts.
- Identified genes represent potential therapeutic targets for managing cataracts.
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