Plasma adriamycin levels during remission induction therapy: RIA versus HPLC

Plasma concentrations of adriamycin and adriamycinol were measured in patients with acute nonlymphocytic leukemia during remission induction therapy with araC adriamycin and cytosine arabinoside. Blood samples were analyzed at 10 min, 1, 3, 6 and 24 hrs after the end of the first, second and third daily injection of adriamycin, using high pressure liquid chromatography and a commercially available radioimmuno assay. Overall, 65 samples were compared, with the correlation coefficient r = 0.765-0.920 for the different time points. The combined correlation coefficient for 57 samples was 0.853. These correlations were significant at the p = 0.005 level.