Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Novel PTPN2 Isoform Differentially Regulates Immune Response.

European journal of immunology·2026
Same author

Unique nasal cell states induced by common pediatric respiratory viruses.

bioRxiv : the preprint server for biology·2026
Same author

Stem bending reduces internode elongation and enhances stem diameter in tomato, with potential involvement of jasmonate-gibberellin and jasmonate-abscisic acid signaling.

Plant physiology and biochemistry : PPB·2026
Same author

Disparities in Admissions for RSV: A Retrospective Observational Study.

Pediatric emergency care·2026
Same author

Ribonucleotide reductase M1 regulates the cell cycle via minichromosome maintenance proteins.

Genes & diseases·2026
Same author

Targeting MLCK1 uncouples immune checkpoint inhibitor-induced colitis from antitumour immunity.

Gut·2026
Same journal

Conserved 3' stem-loop structures enable comprehensive analysis of bacterial transcription termination in metagenomes.

Microbiome·2026
Same journal

Correction: Heat stress suppresses lactation through potential rumen-mammary communication mediated by extracellular vesicles: integrated analysis of microbiome, metabolome, and miRNA profiles.

Microbiome·2026
Same journal

Museomics reveals uncultured symbionts with biosynthetic potential in nudibranchs.

Microbiome·2026
Same journal

Population-based characterisation of child and adolescent oral bacterial microbiomes.

Microbiome·2026
Same journal

Uncovering transcriptional processes in microbial communities adapted to differing saline conditions in salt-weathered historic buildings.

Microbiome·2026
Same journal

IL-17A deficiency in HLA-DR3 transgenic mice enriches beneficial Prevotella species in gut to promote Tregs and reduce CNS autoimmunity.

Microbiome·2026
See all related articles

Related Experiment Video

Updated: Oct 14, 2025

Investigating Intestinal Inflammation in DSS-induced Model of IBD
08:43

Investigating Intestinal Inflammation in DSS-induced Model of IBD

Published on: February 1, 2012

67.8K

Utilizing a reductionist model to study host-microbe interactions in intestinal inflammation.

Amy M Tsou1,2,3,4, Jeremy A Goettel5,6,7, Bin Bao5,6

  • 1Division of Gastroenterology, Hepatology and Nutrition, Boston Children's Hospital, Boston, MA, USA. amt9052@med.cornell.edu.

Microbiome
|November 4, 2021
PubMed
Summary
This summary is machine-generated.

The gut microbiome

Keywords:
Defined consortiumGut microbiotaImmune dysregulationIntestinal inflammationPathobiontWiskott-Aldrich syndrome

More Related Videos

An Intestinal Gut Organ Culture System for Analyzing Host-Microbiota Interactions
05:27

An Intestinal Gut Organ Culture System for Analyzing Host-Microbiota Interactions

Published on: June 30, 2021

4.6K
Development of an Antigen-driven Colitis Model to Study Presentation of Antigens by Antigen Presenting Cells to T Cells
06:57

Development of an Antigen-driven Colitis Model to Study Presentation of Antigens by Antigen Presenting Cells to T Cells

Published on: September 18, 2016

9.2K

Related Experiment Videos

Last Updated: Oct 14, 2025

Investigating Intestinal Inflammation in DSS-induced Model of IBD
08:43

Investigating Intestinal Inflammation in DSS-induced Model of IBD

Published on: February 1, 2012

67.8K
An Intestinal Gut Organ Culture System for Analyzing Host-Microbiota Interactions
05:27

An Intestinal Gut Organ Culture System for Analyzing Host-Microbiota Interactions

Published on: June 30, 2021

4.6K
Development of an Antigen-driven Colitis Model to Study Presentation of Antigens by Antigen Presenting Cells to T Cells
06:57

Development of an Antigen-driven Colitis Model to Study Presentation of Antigens by Antigen Presenting Cells to T Cells

Published on: September 18, 2016

9.2K

Area of Science:

  • Immunology
  • Microbiology
  • Gastroenterology

Background:

  • Altered gut microbiome is linked to inflammatory bowel disease (IBD).
  • Host-microbe interactions in IBD pathogenesis are not fully understood.
  • Wiskott-Aldrich syndrome protein (WASP)-deficient mice develop spontaneous colitis, mimicking human WAS.

Purpose of the Study:

  • Investigate the role of host-microbe interactions in intestinal inflammation using a reductionist model.
  • Determine how specific gut bacteria influence colitis development in immune-dysregulated hosts.
  • Analyze bacterial adaptation and immunogenicity in the context of host immune status.

Main Methods:

  • Utilized Wiskott-Aldrich syndrome protein (WASP)-deficient (Was-/-) and wild-type (WT) mice.
  • Colonized mice with specific gut bacteria: altered Schaedler flora (ASF) and Helicobacter.
  • Employed multi-omics approaches, including meta-transcriptomics and fecal lipocalin-2 (LCN2) analysis.

Main Results:

  • Colitis developed in Was-/- mice colonized with both ASF and Helicobacter, but not with either alone.
  • Helicobacter abundance correlated with inflammation marker LCN2 in Was-/- mice, but inversely in WT mice.
  • Specific bacteria like Mucispirillum schaedleri (ASF457) showed increased immunogenicity in Was-/- mice, while Parabacteroides goldsteinii (ASF519) showed reduced stress adaptation.

Conclusions:

  • Microbial impact on the immune system is context-dependent, varying with host immune status.
  • Bacteria can alter gene expression to enhance fitness and immunogenicity in inflamed, immune-dysregulated environments.
  • Findings emphasize the importance of host-microbe interactions and bacterial adaptation in IBD and microbiota-based therapeutics.