Related Experiment Video
Updated: Oct 14, 2025

Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy
Published on: September 16, 2022
Holographic microscope and its biological application
Xiangyu Quan1, Daisuke Kato2, Vincent Daria3
1Department of System Science, Kobe University Graduate School of System Informatics, Kobe, Japan.
Abstract:
Holographic structured illumination combined with optogenetics enables patterned stimulation of neurons and glial cells in an intact living brain. Moreover, in vivo functional imaging of cellular activity with recent advanced microscope technologies allows for visualization of the cellular responses during learning, emotion and cognition. Integrating these techniques can be used to verify the link between cell function and behavior output. However, there are technical limitations to stimulate multiple cells with high spatial and temporal resolution with available techniques of optogenetic stimulation. Here, we summarized a two-photon microscope combined with holographic system to stimulate multiple cells with high spatial and temporal resolution for living mice and their biological application.
Related Concept Videos
Imaging Biological Samples with Optical Microscopy
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Three-Dimensional Microscopy in Microbiology
Two-Dimensional Microscopy in Microbiology
Overview of Microscopy Techniques
Overview of Electron Microscopy
Confocal Fluorescence Microscopy

