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Updated: Oct 14, 2025

Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species
Published on: October 15, 2013
A pseudo-outbreak of Burkholderia cepacia complex in a Kentucky hospital
Chad C Eldridge1, Andrea H Flinchum1, Doug Thoroughman2
1Healthcare-Associated Infection/Antibiotic Resistance Prevention Program, Division of Epidemiology and Health Planning, Kentucky Department for Public Health, Frankfort, KY.
Abstract:
A cluster of Burkholderia cepacia complex cases from January to October 2020 among outpatients undergoing urologic procedures within a Kentucky hospital's operating rooms was investigated. This investigation included a laboratory look-back, chart reviews, exposure tracing, staff interviews, and direct observation of infection prevention and control practices. A significant protocol breach in a laboratory procedure led to contamination of surgical specimens submitted for culture with nonsterile saline. Pseudo-outbreaks often highlight gaps in infection control processes. Healthcare facilities can make substantial improvements in patient care quality and safety as they respond to identified gaps and improve systems and protocols.
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