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Related Experiment Video

Updated: Oct 14, 2025

Low-cost Custom Fabrication and Mode-locked Operation of an All-normal-dispersion Femtosecond Fiber Laser for Multiphoton Microscopy

Low-cost Custom Fabrication and Mode-locked Operation of an All-normal-dispersion Femtosecond Fiber Laser for Multiphoton Microscopy

Published on: November 22, 2019

Femtosecond diode-based time lens laser for multiphoton microscopy.

Y Lange Simmons¹, Kenneth J Underwood¹, Omkar D Supekar^2,3

¹Department of Physics, University of Colorado, Boulder, CO 80309, USA.

Biomedical Optics Express

|November 8, 2021

Summary

This summary is machine-generated.

We developed a powerful near-infrared diode laser for deep tissue two-photon microscopy. This compact, kW peak power system enables clearer imaging of biological samples at greater depths.

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Last Updated: Oct 14, 2025

Low-cost Custom Fabrication and Mode-locked Operation of an All-normal-dispersion Femtosecond Fiber Laser for Multiphoton Microscopy

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Published on: November 22, 2019

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Photostimulation by Femtosecond Laser Activates Extracellular-signal-regulated Kinase ERK Signaling or Mitochondrial Events in Target Cells

Photostimulation by Femtosecond Laser Activates Extracellular-signal-regulated Kinase ERK Signaling or Mitochondrial Events in Target Cells

Published on: July 6, 2019

Area of Science:

Biomedical optics
Microscopy technology
Laser physics

Background:

Two-photon microscopy (TPM) is crucial for deep tissue imaging.
Existing laser sources can be bulky, inefficient, or lack sufficient peak power.
Advancements in laser technology are needed to improve accessibility and performance in TPM.

Purpose of the Study:

To demonstrate a novel near-infrared, femtosecond, diode laser source.
To achieve kilowatt (kW) peak power for enhanced two-photon microscopy.
To enable deeper and more efficient imaging of biological tissues.

Main Methods:

Utilized a diode laser-based system emitting at 976 nm.
Generated sub-picosecond (sub-ps) pulses at a 10 MHz repetition rate.
Integrated the laser source with a laser-scanning microscope.

Main Results:

Achieved kW class peak powers suitable for deep tissue imaging.
Successfully imaged to a depth of 900 µm in fixed mouse brain tissue (PLP-eGFP labeled).
Demonstrated the system's capability for high-resolution deep tissue visualization.

Conclusions:

The developed diode laser source offers a significant advancement for two-photon microscopy.
This technology leads to more efficient, compact, and accessible laser systems for biomedical imaging.
The system's performance supports improved diagnostic and research capabilities in deep tissue imaging.