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Updated: Oct 14, 2025
Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell Culture
Published on: February 23, 2018
Quantitative evaluation of the dynamic activity of HeLa cells in different viability states using dynamic full-field
Soongho Park1, Thien Nguyen1, Emilie Benoit2
1Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, 49 Convent Dr., Bethesda 20814, USA.
Abstract:
Dynamic full-field optical coherence microscopy (DFFOCM) was used to characterize the intracellular dynamic activities and cytoskeleton of HeLa cells in different viability states. HeLa cell samples were continuously monitored for 24 hours and compared with histological examination to confirm the cell viability states. The averaged mean frequency and magnitude observed in healthy cells were 4.79±0.5 Hz and 2.44±1.06, respectively. In dead cells, the averaged mean frequency was shifted to 8.57±0.71 Hz, whereas the magnitude was significantly decreased to 0.53±0.25. This cell dynamic activity analysis using DFFOCM is expected to replace conventional time-consuming and biopsies-required histological or biochemical methods.
