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Related Experiment Video

Updated: Oct 14, 2025

Isolation of Murine Lymph Node Stromal Cells

Isolation of Murine Lymph Node Stromal Cells

Published on: August 19, 2014

Processing human lymph node samples for single-cell assays.

Tobias Roider^1,2,3, Berit J Brinkmann^1,2,3,4, Sascha Dietrich^1,2,3

¹European Molecular Biology Laboratory (EMBL), 69120 Heidelberg, Germany.

|November 8, 2021

Summary

This summary is machine-generated.

This study presents a gentle, time-efficient protocol for processing human lymph node samples. The method yields single-cell suspensions for advanced analyses like single-cell RNA sequencing in lymphoma research.

Keywords:

Cancer Cell isolation Flow Cytometry/Mass Cytometry Health Sciences Single Cell

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Last Updated: Oct 14, 2025

Isolation of Murine Lymph Node Stromal Cells

Isolation of Murine Lymph Node Stromal Cells

Published on: August 19, 2014

Immunostimulatory Agent Evaluation: Lymphoid Tissue Extraction and Injection Route-Dependent Dendritic Cell Activation

Immunostimulatory Agent Evaluation: Lymphoid Tissue Extraction and Injection Route-Dependent Dendritic Cell Activation

Published on: September 16, 2018

Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting

Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting

Published on: May 1, 2015

Area of Science:

Oncology
Immunology
Cell Biology

Background:

Non-Hodgkin's lymphoma (NHL) predominantly resides within lymph nodes, shielded by the tumor microenvironment.
Understanding cellular heterogeneity and cell interactions in NHL requires patient-derived samples.
Delicate assays necessitate high-quality single-cell suspensions from lymph node tissue.

Purpose of the Study:

To develop a rapid and gentle protocol for processing human lymph node samples.
To generate single-cell suspensions suitable for sensitive downstream applications.
To facilitate the study of cellular heterogeneity and cell-cell interactions in lymphoma.

Main Methods:

A novel protocol for human lymph node sample processing is described.
The method avoids enzymatic digestion and mechanical stress.
Optimization focused on preserving cellular integrity for single-cell analysis.

Main Results:

The protocol is time-efficient and gentle on delicate cells.
It successfully produces single-cell suspensions from lymph node tissue.
The resulting suspensions are suitable for assays like single-cell RNA sequencing.

Conclusions:

This protocol offers an effective means to prepare patient-derived lymph node samples.
It supports advanced single-cell analyses crucial for lymphoma research.
The method aids in investigating the tumor microenvironment and cellular heterogeneity in NHL.