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Updated: Oct 14, 2025

Monitoring Stub1-Mediated Pexophagy
Published on: May 12, 2023
Ubiquitin ligase MARCH5 localizes to peroxisomes to regulate pexophagy
Jun Zheng1,2,3, Xi Chen1, Qiang Liu4
1School of Life Science and Technology, ShanghaiTech University, Shanghai, China.
Abstract:
Mitochondria and peroxisomes are independent but functionally closely related organelles. A few proteins have been characterized as dual-organelle locating proteins with distinct or similar roles on mitochondria and peroxisomes. MARCH5 is a mitochondria-associated ubiquitin ligase best known for its regulatory role in mitochondria quality control, fission, and fusion. Here, we used a proximity tagging system, PUP-IT, and identified new interacting proteins of MARCH5. Our data uncover that MARCH5 is a dual-organelle locating protein that interacts with several peroxisomal proteins. PEX19 binds the transmembrane region on MARCH5 and targets it to peroxisomes. On peroxisomes, MARCH5 binds and mediates the ubiquitination of PMP70. Furthermore, we find PMP70 ubiquitination and pexophagy induced by mTOR inhibition are blocked in the absence of MARCH5. Our study suggests novel roles of MARCH5 on peroxisomes.
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