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Related Experiment Video

Updated: Oct 14, 2025

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

Nanopore sequencing technology, bioinformatics and applications.

Yunhao Wang¹, Yue Zhao^1,2, Audrey Bollas¹

¹Department of Biomedical Informatics, The Ohio State University, Columbus, OH, USA.

Nature Biotechnology

|November 9, 2021

Summary

Nanopore sequencing technology has rapidly advanced, improving DNA and RNA analysis for genomes and transcriptomes. Further development will enhance data quality and analytical methods for diverse applications.

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Last Updated: Oct 14, 2025

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

Nanopore DNA Sequencing for Metagenomic Soil Analysis

Nanopore DNA Sequencing for Metagenomic Soil Analysis

Published on: December 14, 2017

Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

Published on: March 11, 2020

Area of Science:

Genomics and Molecular Biology
Bioinformatics and Computational Biology

Background:

Nanopore sequencing technology has seen rapid advancements.
Improvements in accuracy, read length, and throughput are notable.

Purpose of the Study:

To highlight the extensive development of experimental and bioinformatics methods for nanopore long reads.
To showcase the diverse applications of nanopore sequencing.

Main Methods:

Utilizing nanopore sequencing for long DNA and RNA molecules.
Developing advanced bioinformatics pipelines for data analysis.

Main Results:

Nanopore sequencing is applied in genome assembly, full-length transcript detection, and base modification detection.
Specialized applications include clinical diagnostics and outbreak surveillance.

Conclusions:

Significant opportunities exist for enhancing data quality and analytical approaches.
Future developments in nanopores, base-calling, and experimental protocols are crucial.