Photochemical Disulfide-Ene Modification Enhances Protein Sequencing and Disulfide Mapping by Mass Spectrometry
Xiaoyue Yang1, Longfei Zhang1, Yu Xia1
1Department of Chemistry, Tsinghua University, Beijing 100084, China.
Analytical Chemistry
|November 9, 2021
Abstract:
A new photochemical disulfide-ene reaction system capable of alkylating protein disulfide bonds in seconds has been established. The system is simple, containing acetone and isopropanol for disulfide reduction under 254 nm UV irradiation and norbornene as a highly efficient alkylation reagent. Enhanced characterization of disulfide-rich proteins with significantly shortened analysis time is demonstrated by coupling the reaction online with mass spectrometry.


