Responsive-released strategy based on lead ions-dependent DNAzyme functionalized UIO-66-NH₂ for tumor marker

Area of Science:

• * Electrochemistry
• * Materials Science
• * Biomedical Engineering

Background:

• * Electrode interface signal labeling is crucial for immunosensors but faces challenges like flimsy methods and protein interference.
• * Existing electrochemical immunoassay methods require improvement for accurate signal readout.

Purpose of the Study:

• * To develop a lead ion-decodable signal integrator based on UIO-66-NH2 for prostate specific antigen (PSA) detection.
• * To overcome limitations of traditional signal labeling in electrochemical immunoassays.

Main Methods:

• * A lead ion-dependent DNAzyme functionalized UIO-66-NH2, encapsulating methylene blue, was synthesized.
• * The UIO-66-NH2 was dispersed in solution and associated with lead sulfide labeled sandwich bioconjugates.
• * A cationic exchange reaction between lead sulfide and silver ions triggered methylene blue release, detected electrochemically.

Main Results:

• * The immunoassay demonstrated a wide linear range for PSA detection from 1 pg mL⁻¹ to 10 ng mL⁻¹.
• * A sensitive detection limit of 0.34 pg mL⁻¹ was achieved.
• * The strategy effectively linked PSA immunoassay with methylene blue release dynamics via DNAzyme cleavage on MOFs.

Conclusions:

• * The proposed UIO-66-NH2 based signal integrator offers an effective strategy for electrochemical immunoassays.
• * This approach shows promise for early and accurate cancer diagnosis through improved immunosensor technology.
• * The study highlights the potential of Metal-Organic Frameworks (MOFs) in advanced diagnostic applications.