Related Experiment Video

Updated: Oct 13, 2025

Implementing Patch Clamp and Live Fluorescence Microscopy to Monitor Functional Properties of Freshly Isolated PKD Epithelium
08:46

Implementing Patch Clamp and Live Fluorescence Microscopy to Monitor Functional Properties of Freshly Isolated PKD Epithelium

Published on: September 1, 2015

9.9K

"ADPKD-omics": determinants of cyclic AMP levels in renal epithelial cells

Yash R Mehta1, Spencer A Lewis1, Kirby T Leo1

  • 1Epithelial Systems Biology Laboratory, Systems Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland, USA.

Kidney International
|November 10, 2021
PubMed

Insights

Cyclic adenosine monophosphate (cAMP) regulation in kidney cells impacts water balance and autosomal dominant polycystic kidney disease (ADPKD). This study identifies key proteins controlling cAMP levels in kidney tubules, aiding ADPKD research.

Area of Science:

  • Nephrology
  • Molecular Biology
  • Genetics

Background:

  • Cyclic adenosine monophosphate (cAMP) signaling is crucial for kidney function.
  • Dysregulation of cAMP is implicated in water balance disorders and autosomal dominant polycystic kidney disease (ADPKD).

Purpose of the Study:

  • To identify and characterize proteins that regulate cAMP levels in kidney epithelial cells.
  • To determine the expression of these cAMP-regulating proteins across kidney tubule segments.
  • To provide a resource for understanding ADPKD pathogenesis.

Main Methods:

  • Review of genes encoding cAMP level determinants.
  • Analysis of RNA-sequencing and protein mass spectrometry data for 14 kidney tubule segments.
  • Integration of "autosomal dominant polycystic kidney disease-omics" data.

Main Results:

  • A comprehensive list of cAMP-level determinants, including G protein-coupled receptors, adenylyl cyclases, and phosphodiesterases, was compiled.
  • Expression patterns of these proteins across kidney tubule segments were identified.
  • A specialized database of the primary cilium proteome was created.

Conclusions:

  • This curated list of proteins provides a valuable resource for understanding cAMP regulation in kidney epithelial cells.
  • Identifying these determinants may offer insights into the progression of autosomal dominant polycystic kidney disease.
  • Compartmentalized cAMP signaling in primary cilia is highlighted as an area for further investigation.
Keywords:
RNA sequencingprotein mass spectrometryvaptansvasopressin

More Related Videos

Isolation, Characterization, And High Throughput Extracellular Flux Analysis of Mouse Primary Renal Tubular Epithelial Cells09:40

Isolation, Characterization, And High Throughput Extracellular Flux Analysis of Mouse Primary Renal Tubular Epithelial Cells

Published on: June 20, 2018

18.0K
Measurement of 3-Dimensional cAMP Distributions in Living Cells using 4-Dimensional x, y, z, and λ Hyperspectral FRET Imaging and Analysis08:22

Measurement of 3-Dimensional cAMP Distributions in Living Cells using 4-Dimensional x, y, z, and λ Hyperspectral FRET Imaging and Analysis

Published on: October 27, 2020

4.0K

Related Concept Videos

cAMP-dependent Protein Kinase Pathways01:25

cAMP-dependent Protein Kinase Pathways

Cyclic Adenosine Monophosphate (cAMP) is an essential second messenger that activates protein kinase A (PKA) and regulates various biological processes. A single epinephrine molecule binds to GPCR and activates several heterotrimeric G proteins, each stimulating multiple adenylyl cyclase, amplifying the signal, and synthesizing large numbers of cAMP molecules. Small changes in cAMP concentration affect PKA activity. The binding of four cAMP molecules induces a conformational change in PKA,...
6.9K
Intracellular Signaling Cascades01:24

Intracellular Signaling Cascades

Once a ligand binds to a receptor, the signal is transmitted through the membrane and into the cytoplasm. The continuation of a signal in this manner is called signal transduction. Signal transduction only occurs with cell-surface receptors, which cannot interact with most components of the cell, such as DNA. Only internal receptors can interact directly with DNA in the nucleus to initiate protein synthesis. When a ligand binds to its receptor, conformational changes occur that affect the...
48.7K
Global Regulatory Systems01:28

Global Regulatory Systems

Global regulatory systems in bacteria enable rapid and coordinated responses to environmental changes by integrating sensory inputs with gene expression, ensuring efficient adaptation to fluctuating conditions. Key global regulatory mechanisms include regulons, two-component systems, sigma factors, and secondary messengers.Regulons and Global RegulatorsA regulon is a collection of genes and operons controlled by a common global regulator. These regulators enable bacteria to prioritize resource...
136
GPCRs Regulate Adenylyl Cylase Activity01:09

GPCRs Regulate Adenylyl Cylase Activity

Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of...
6.1K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
14.2K
Endocrine Signaling01:45

Endocrine Signaling

Endocrine cells produce hormones to communicate with remote target cells found in other organs. The hormone reaches these distant areas using the circulatory system. This exposes the whole organism to the hormone but only those cells expressing hormone receptors or target cells are affected. Thus, endocrine signaling induces slow responses from its target cells but these effects also last longer.
65.5K