Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Phase Engineering of Iridium Oxides Enables Direct Coupling of Proton Exchange Membrane Water Electrolysis With Intermittent Electrical Energy.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Corrigendum to "Xanthoxylin alleviates dextran sulfate sodium (DSS)-induced colitis by targeting macrophage infiltration via the tumor necrosis factor (TNF)/nuclear factor-kappa B (NF-κB) signaling pathway" [Phytomedicine 153:157971 (2026) PMID: 41720014].

Phytomedicine : international journal of phytotherapy and phytopharmacology·2026
Same author

AdaptCMVC++: Robust and Flexible Adaptation to Incremental Views in Continual Multi-view Clustering.

IEEE transactions on pattern analysis and machine intelligence·2026
Same author

Combating Inflammation and Promoting Anabolism in Osteoarthritic Cartilage Defect With an MMP13-Sensing Dual-Drug Scaffold.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Rapid and real-time detection of trace BPA based on solution-gated graphene field-effect transistor by cerium-doped ZIF-8 carbon material.

Talanta·2026
Same author

A Multi-Regional Single-nucleus Atlas of the Huntington's Disease Brain.

Scientific data·2026

Related Experiment Video

Updated: Oct 13, 2025

Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase
10:01

Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase

Published on: December 4, 2017

12.4K

A phase-transfer catalyst-based nanoreactor for accelerated hydrogen sulfide bio-imaging.

Panfei Xing1,2, Yiming Niu1,3, Jiacheng Li1

  • 1State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Taipa, Macau SAR, China. cmwang@umac.mo.

Nanoscale
|November 10, 2021
PubMed
Summary

Researchers developed a new phase-transfer catalyst (PTC) approach to create faster fluorescent nanoprobes for detecting hydrogen sulfide (H2S) in living cells. These nanoprobes significantly reduce detection time, enabling real-time biological imaging.

More Related Videos

Synthesis of Metal Nanoparticles Supported on Carbon Nanotube with Doped Co and N Atoms and its Catalytic Applications in Hydrogen Production
08:40

Synthesis of Metal Nanoparticles Supported on Carbon Nanotube with Doped Co and N Atoms and its Catalytic Applications in Hydrogen Production

Published on: December 6, 2021

3.8K
A Continuous-flow Photocatalytic Reactor for the Precisely Controlled Deposition of Metallic Nanoparticles
11:49

A Continuous-flow Photocatalytic Reactor for the Precisely Controlled Deposition of Metallic Nanoparticles

Published on: April 10, 2019

9.9K

Related Experiment Videos

Last Updated: Oct 13, 2025

Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase
10:01

Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase

Published on: December 4, 2017

12.4K
Synthesis of Metal Nanoparticles Supported on Carbon Nanotube with Doped Co and N Atoms and its Catalytic Applications in Hydrogen Production
08:40

Synthesis of Metal Nanoparticles Supported on Carbon Nanotube with Doped Co and N Atoms and its Catalytic Applications in Hydrogen Production

Published on: December 6, 2021

3.8K
A Continuous-flow Photocatalytic Reactor for the Precisely Controlled Deposition of Metallic Nanoparticles
11:49

A Continuous-flow Photocatalytic Reactor for the Precisely Controlled Deposition of Metallic Nanoparticles

Published on: April 10, 2019

9.9K

Area of Science:

  • Biochemistry
  • Chemical Biology
  • Cell Biology

Background:

  • Hydrogen sulfide (H2S) is a crucial signaling molecule involved in numerous biological processes.
  • Current fluorescent probes for H2S detection in living cells suffer from long reaction times, limiting real-time monitoring.
  • Hydrophobic small molecule fluorescent probes require efficient strategies for use in aqueous biological environments.

Purpose of the Study:

  • To develop a novel phase-transfer catalyst (PTC) approach for rapid H2S detection in living cells.
  • To create amphiphilic H2S nanoprobes with accelerated reaction kinetics for real-time biological imaging.
  • To synthesize and evaluate new fluorescent nanoprobes for sensitive and fast H2S sensing.

Main Methods:

  • Conjugation of common fluorescent probes with amphiphilic PEG-PPG-PEG polymers to form nanoprobes.
  • Utilizing a phase-transfer catalyst (PTC) microenvironment within the nanoprobes to accelerate analyte reactions.
  • Synthesis of two distinct wavelength nanoprobes (DS-Blue-nano and DN-Green-nano) for H2S detection.
  • Testing nanoprobes in living macrophage cells for real-time H2S imaging.

Main Results:

  • The PTC approach significantly reduced H2S detection time to 3-10 minutes, compared to 20-60 minutes for conventional probes.
  • Developed nanoprobes (DS-Blue-nano and DN-Green-nano) demonstrated sensitive H2S detection in living cells.
  • Bright fluorescence signals were observed as early as 7 minutes, reaching stability by 15 minutes in cellular imaging.
  • The nanoprobes enabled real-time imaging of H2S dynamics in biological systems.

Conclusions:

  • The PTC-based strategy provides a generic and effective method for developing rapid fluorescent probes.
  • This approach enables sensitive, real-time imaging of hydrogen sulfide in living cells.
  • The developed nanoprobes offer a significant advancement for studying biological processes involving H2S.