Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Sulfur-Substituted SAMs Induce Pb─S Antibonding Hybridization for Efficient and Durable Perovskite-Silicon Tandems.

Advanced materials (Deerfield Beach, Fla.)·2026

Same author

Diagnostic efficacy of circulating tumor cell in clinically significant prostate cancer.

Scientific reports·2026

Same author

[Embryonic Stem Cell-Derived Mesenchymal Stromal Cell Exosomes Protect Against Radiation-Induced Lymphocyte Injury and Its Related Mechanisms].

Zhongguo shi yan xue ye xue za zhi·2026

Same author

HOPX is required for the generation of umbilical cord blood-derived memory-like NK cells induced by three cytokines.

Frontiers in immunology·2026

Same author

Research on the Determination Method of Additional Safety Factor Margin for Ultradeep Well Pipe Strings under Multisource Loads.

ACS omega·2026

Same author

Machine Learning Accelerated Non-Adiabatic Molecular Dynamics Elucidates Local Polarization Effects on Non-radiative Recombination in Halide Perovskites.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Search research articles

Related Experiment Video

Updated: Oct 13, 2025

Author Spotlight: Characterizing Novel Enzymes from Extremophiles and Common Pathogens to Understand DNA Repair and Replication

Author Spotlight: Characterizing Novel Enzymes from Extremophiles and Common Pathogens to Understand DNA Repair and Replication

Published on: July 5, 2024

Multiplexed Small-Molecule-Ligand Binding Assays by Affinity Labeling and DNA Sequence Analysis.

Bo Cai¹, Casey J Krusemark¹

¹Department of Medicinal Chemistry and Molecular Pharmacology, Purdue Center for Cancer Research, Purdue University, West Lafayette, IN, 47907, USA.

Angewandte Chemie (International Ed. in English)

|November 10, 2021

Summary

We developed a novel DNA-based assay for drug discovery that improves cost, sensitivity, and throughput. This method enables high-throughput screening of small molecules by analyzing DNA barcodes after protein binding.

Keywords:

affinity labelling drug discovery ligand binding assays protein-ligand interactions

More Related Videos

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

Sequence-specific Labeling of Nucleic Acids and Proteins with Methyltransferases and Cofactor Analogues

Sequence-specific Labeling of Nucleic Acids and Proteins with Methyltransferases and Cofactor Analogues

Published on: November 22, 2014

Related Experiment Videos

Last Updated: Oct 13, 2025

Author Spotlight: Characterizing Novel Enzymes from Extremophiles and Common Pathogens to Understand DNA Repair and Replication

Author Spotlight: Characterizing Novel Enzymes from Extremophiles and Common Pathogens to Understand DNA Repair and Replication

Published on: July 5, 2024

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

Sequence-specific Labeling of Nucleic Acids and Proteins with Methyltransferases and Cofactor Analogues

Sequence-specific Labeling of Nucleic Acids and Proteins with Methyltransferases and Cofactor Analogues

Published on: November 22, 2014

Area of Science:

Biochemistry
Molecular Biology
Drug Discovery

Background:

Small-molecule protein binding assays are crucial for drug discovery but face limitations in cost, sensitivity, and throughput.
Existing assay formats often require complex procedures and can be inefficient for large-scale screening.

Purpose of the Study:

To develop an improved assay method for small-molecule binding to target proteins.
To enhance assay sensitivity, cost-effectiveness, and throughput using DNA sequence analysis.

Main Methods:

Developed a novel assay combining DNA encoding with split-and-pool sample handling.
Utilized affinity labeling of DNA-linked ligands to protein targets.
Employed purifying selection and DNA barcode quantification to detect ligand binding.

Main Results:

Demonstrated the assay's utility in both ligand displacement and direct binding formats.
Successfully determined relative ligand affinity and profiled ligand specificity.
Validated the approach for high-throughput small-molecule screening.

Conclusions:

The developed DNA-based assay offers a sensitive, cost-effective, and high-throughput solution for drug discovery.
This method facilitates efficient screening of small molecules for protein targets.
The assay provides a powerful tool for determining ligand binding characteristics.