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TSH receptor structure.

J Chan, P Santisteban, M De Luca

    Acta Endocrinologica. Supplementum
    |January 1, 1987
    PubMed
    Summary
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    Thyroid stimulating hormone (TSH) receptor proteins in rat thyroid cells were analyzed. TSH influences the synthesis and degradation of these receptor proteins, affecting their molecular weight and presence.

    Area of Science:

    • Endocrinology
    • Molecular Biology
    • Cell Biology

    Background:

    • The TSH receptor mediates the action of TSH in thyroid cells.
    • Understanding TSH receptor dynamics is crucial for thyroid function and disease.

    Purpose of the Study:

    • To investigate the molecular characteristics and regulation of the TSH receptor in FRTL-5 rat thyroid cells.
    • To determine how TSH influences the synthesis and degradation of TSH receptor-related proteins.

    Main Methods:

    • Solubilization and TSH affinity chromatography of radiolabeled membrane preparations.
    • Immunoprecipitation using monoclonal antibodies against the TSH receptor.
    • In vitro protein derivation and translation experiments.
    • Analysis of ganglioside composition.

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    Main Results:

    • Two distinct TSH receptor protein peaks (approx. 300 K and 70 K) were identified, with the 70 K protein derived from the 300 K protein.
    • TSH stimulation increased the synthesis and degradation of these proteins, leading to multiple immunoprecipitable bands.
    • A specific disialo ganglioside containing fucose was found in the TSH receptor fractions.
    • mRNA translation experiments identified a single component producing a ~220 K protein, absent in cells lacking functional TSH receptors.

    Conclusions:

    • TSH regulates the TSH receptor through modulation of synthesis and degradation pathways.
    • The TSH receptor exists in different molecular forms and is associated with specific gangliosides.
    • A ~220 K protein is a key product of TSH receptor mRNA, indicative of receptor functionality.