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Related Experiment Videos

Ascorbic acid metabolism in diabetes mellitus.

R E Pecoraro, M S Chen

    Annals of the New York Academy of Sciences
    |January 1, 1987
    PubMed
    Summary

    Acute glucose administration lowers ascorbic acid (AA) in leukocytes but does not immediately affect cell movement. Prolonged high blood sugar impairs leukocyte function, potentially impacting infection and healing in diabetes.

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    Area of Science:

    • Biochemistry
    • Immunology
    • Cell Biology

    Background:

    • Competition for membrane transport exists between glucose and ascorbic acid (AA).
    • In vitro studies show this competition in human lymphocytes, granulocytes, and fibroblasts.

    Purpose of the Study:

    • To investigate the effects of acute intravenous glucose administration on AA levels in mononuclear (MNL) and polymorphonuclear leukocytes (PMN).
    • To assess the impact of glucose on leukocyte chemotaxis.
    • To explore the relationship between chronic hyperglycemia, intracellular AA, and inflammatory responses.

    Main Methods:

    • Measurement of plasma glucose and AA, MNL AA, PMN AA, and leukocyte chemotaxis before and after i.v. glucose in fasted volunteers.
    • Utilized a glucose clamp technique to maintain prolonged hyperglycemia.
    • Analyzed changes in chemotaxis correlated with intracellular AA levels.

    Main Results:

    • A decline in AA was observed in both PMN and MNL following acute glucose administration.
    • Acute hyperglycemia did not significantly alter leukocyte chemotaxis.
    • Prolonged hyperglycemia (210-240 min) led to significant changes in PMN and MNL chemotaxis.
    • Decreased intracellular AA levels after 240 min correlated with altered chemotaxis to specific chemoattractants.

    Conclusions:

    • Acute glucose administration transiently reduces leukocyte AA levels without affecting chemotaxis.
    • Chronic hyperglycemia may lead to intracellular AA deficits in leukocytes.
    • These deficits are associated with impaired inflammatory responses, potentially increasing susceptibility to infection and hindering wound repair in diabetic patients.

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