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Updated: Oct 13, 2025

Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain
Published on: March 13, 2020
Untargeted Metabolomic Profiling Reveals Variation in Metabolites Associated with Nutritional Values in Tef
Aiswarya Girija1, Rattan Yadav1, Fiona Corke1
1Institute of Biological, Environmental and Rural Studies (IBERS), Aberystwyth University, Aberystwyth, SY23 3DA, Ceredigion, UK.
Abstract:
Tef (Eragrostis tef), is a gluten-free orphan cereal, crop of nutritional and economical significance. Here we used untargeted metabolomics to survey metabolite variation in 14 diverse tef accessions at 15-days post germination. Tef genotypes were classified into four metabolomic groups where variation was linked to flavones and flavonols. Further analysis on white seeded accessions shows variation related to sucrose and important vitamins, nicotinamides (vitamin B3) riboflavin (vitamin B2) and folate (vitamin B9). Coloured seeded accessions showed variation in metabolism related to amino acid and sugars. This study highlights the potential of metabolomics in exploring the nutritional traits in tef.

