Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Scanning Electron Microscopy01:07

Scanning Electron Microscopy

4.5K
A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
4.5K
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

13.7K
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
13.7K
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.6K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.6K
Overview of Electron Microscopy01:25

Overview of Electron Microscopy

11.7K
The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
11.7K
Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

6.0K
To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
6.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

BAP1-tumour predisposition syndrome.

The British journal of dermatology·2026
Same author

Democratized single-cell proteomics resolves cell state heterogeneity in skin tumors.

Life science alliance·2026
Same author

Vision-related quality of life in non-neovascular age-related macular degeneration: a protocol for systematic review and meta-analysis.

Eye (London, England)·2026
Same author

The importance of challenging dogma with patient data.

The Journal of investigative dermatology·2026
Same author

When less is more: The role of dermatologists in promoting societal and self-acceptance of skin health and disease.

The British journal of dermatology·2026
Same author

Ninth BHD International Symposium: Advancing research through global collaboration.

Cell stress & chaperones·2026
Same journal

An Integrated Skin Cell Atlas Decodes the Pilosebaceous Unit.

The Journal of investigative dermatology·2026
Same journal

Residual CSB activity explains mild UV-sensitive syndrome phenotype caused by CSB mutations.

The Journal of investigative dermatology·2026
Same journal

Charting a new melanoma risk factor: Satellite Naevus Prevalence in High-Risk and Population-Based Cohorts.

The Journal of investigative dermatology·2026
Same journal

Human keratinocytes and fibroblasts coordinate early cutaneous innate defenses against Candida auris.

The Journal of investigative dermatology·2026
Same journal

Merkel cells attenuate autoantigen-specific T cell driven skin inflammation in mice associated with reduced neutrophil recruitment.

The Journal of investigative dermatology·2026
Same journal

The response of human melanocytic nevi to simulated solar radiation assessed by single-nucleus RNA sequencing of frozen tissue.

The Journal of investigative dermatology·2026
See all related articles

Related Experiment Video

Updated: Oct 13, 2025

Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy
09:47

Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy

Published on: July 15, 2021

5.0K

Research Techniques Made Simple: Volume Scanning Electron Microscopy.

Ross Laws1, David H Steel2, Neil Rajan3

  • 1Electron Microscopy Research Services, Newcastle University, Newcastle upon Tyne, United Kingdom; Translational and Clinical Research Institute, Newcastle University, Newcastle upon Tyne, United Kingdom.

The Journal of Investigative Dermatology
|November 11, 2021
PubMed
Summary
This summary is machine-generated.

Volume scanning electron microscopy (VSEM) enables nanoscale 3D visualization of cellular structures. This technique reveals spatial relationships within tissues, aiding dermatological research by offering detailed insights beyond conventional microscopy methods.

More Related Videos

Author Spotlight: A Three-Dimensional Technique for the Visualization of Mitochondrial Ultrastructural Changes in Pancreatic Cancer Cells
08:46

Author Spotlight: A Three-Dimensional Technique for the Visualization of Mitochondrial Ultrastructural Changes in Pancreatic Cancer Cells

Published on: June 23, 2023

1.8K
Targeted Studies Using Serial Block Face and Focused Ion Beam Scan Electron Microscopy
09:09

Targeted Studies Using Serial Block Face and Focused Ion Beam Scan Electron Microscopy

Published on: August 10, 2019

9.4K

Related Experiment Videos

Last Updated: Oct 13, 2025

Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy
09:47

Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy

Published on: July 15, 2021

5.0K
Author Spotlight: A Three-Dimensional Technique for the Visualization of Mitochondrial Ultrastructural Changes in Pancreatic Cancer Cells
08:46

Author Spotlight: A Three-Dimensional Technique for the Visualization of Mitochondrial Ultrastructural Changes in Pancreatic Cancer Cells

Published on: June 23, 2023

1.8K
Targeted Studies Using Serial Block Face and Focused Ion Beam Scan Electron Microscopy
09:09

Targeted Studies Using Serial Block Face and Focused Ion Beam Scan Electron Microscopy

Published on: August 10, 2019

9.4K

Area of Science:

  • Cell Biology
  • Microscopy Techniques
  • Dermatology

Background:

  • Conventional electron microscopy offers limited 3D structural insights.
  • Nanoscale visualization of cellular and tissue architecture is crucial for understanding biological processes.

Purpose of the Study:

  • To provide an overview of Volume Scanning Electron Microscopy (VSEM) platforms and image processing.
  • To guide the selection of VSEM methods for dermatological research questions.

Main Methods:

  • Serial sectioning and imaging of samples using Scanning Electron Microscopy (SEM).
  • Computer-assisted segmentation and 3D reconstruction of image data.
  • Analysis of qualitative and quantitative properties of cellular structures at the nanoscale.

Main Results:

  • VSEM allows for detailed 3D visualization of organelles and cells within tissues.
  • The technique reveals spatial relationships of structures within and across cells.
  • VSEM provides nanoscale resolution for complex biological structures.

Conclusions:

  • VSEM is a powerful tool for high-resolution 3D structural analysis in biological research.
  • The presented overview aids researchers in choosing appropriate VSEM approaches for dermatological studies.
  • VSEM significantly enhances the understanding of tissue and cellular organization compared to traditional methods.