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Updated: Oct 13, 2025

Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry

Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry

Published on: March 5, 2019

High-Dimensional Immunophenotyping with 37-Color Panel Using Full-Spectrum Cytometry.

Marco A Fernandez¹, Hammad Alzayat¹, Maria C Jaimes²

¹Flow Cytometry Facility, Germans Trias i Pujol Research Institute, Badalona, Spain.

Methods in Molecular Biology (Clifton, N.J.)

|November 12, 2021

Summary

This summary is machine-generated.

This study introduces a novel 37-color flow cytometry protocol for detailed immune cell analysis. It effectively identifies over 45 cell populations in COVID-19 patients, advancing immunophenotyping capabilities.

Keywords:

Deep immunophenotyping Full-spectrum cytometry Spectral signature

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Area of Science:

Immunology
Cell Biology
Biotechnology

Background:

Comprehensive immunophenotyping is crucial for understanding immune system components.
Conventional flow cytometry has limitations in analyzing numerous cell subsets simultaneously.
Novel full-spectrum technology offers enhanced data acquisition per sample.

Purpose of the Study:

To describe an effective and practical protocol for deep and broad immunophenotyping.
To leverage full-spectrum technology for expanded biological insights.
To present a 37-color flow cytometry protocol for analyzing SARS-CoV-2 infected patients' blood samples.

Main Methods:

Development and application of a 37-color flow cytometry panel.
Utilizing full-spectrum technology for maximal dye information.
Analysis of whole blood samples from SARS-CoV-2 infected patients.

Main Results:

Identification of more than 45 distinct immune cell populations.
Demonstration of the protocol's effectiveness in complex biological samples.
Successful application in the context of SARS-CoV-2 infection.

Conclusions:

The 37-color protocol enables comprehensive immunophenotyping.
Full-spectrum technology significantly enhances data output in flow cytometry.
This method provides expanded knowledge of immune responses in SARS-CoV-2 patients.