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Closed-Loop Recycling Dual-Mode Counter-Current Chromatography with Specified Sample Loading Durations: Modeling of
Artak E Kostanyan1, Andrey A Voshkin1
1Kurnakov Institute of General and Inorganic Chemistry, Russian Academy of Sciences, Leninsky Prospekt 31, 119991 Moscow, Russia.
Molecules (Basel, Switzerland)
|November 13, 2021
Summary
Closed-loop recycling dual-mode counter-current chromatography (CLR DM CCC) theory is advanced for high-throughput preparative separations. New equations simulate continuous sample loading for industrial applications.
Area of Science:
- Analytical Chemistry
- Separation Science
- Chromatography
Background:
- Closed-loop recycling dual-mode counter-current chromatography (CLR DM CCC) combines closed-loop recycling (CLR) and dual-mode (DM) CCC.
- Previous work analyzed CLR DM CCC under impulse sample injection conditions.
- This method offers sequential separation using mobile phase X and mobile phase Y.
Purpose of the Study:
- To further develop the theory of CLR DM CCC for preparative and industrial-scale separations.
- To enable high-throughput operation required for large-scale applications.
- To simulate CLR DM CCC separations with continuous sample loading over a defined period.
Main Methods:
- Theoretical analysis of CLR DM CCC implementations.
- Development of equations for simulating separations with continuous sample loading.
- Utilized "Mathcad" software for equation development and presentation.
Main Results:
- Established theoretical framework for CLR DM CCC applied to continuous loading.
- Provided equations enabling simulation of large sample volume loading.
- Demonstrated potential for high-throughput separations in preparative and industrial contexts.
Conclusions:
- The developed theory and simulation equations are crucial for advancing CLR DM CCC.
- This advancement supports the application of CLR DM CCC in industrial-scale chromatographic separations.
- The method is optimized for high-throughput processing of large sample volumes.
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