Related Experiment Video
Updated: Oct 13, 2025
Anesthesia-free Heartbeat Measurements in Freely Moving Zebrafish
Published on: April 18, 2025
Label-free quantitative measurement of cardiovascular dynamics in a zebrafish embryo using
Jeeranan Boonruangkan1, Hamid Farrokhi1, Thazhe M Rohith1
1Nanyang Technological University, School of Mechanical and Aerospace Engineering, Singapore.
Significance:
Real-time monitoring of the heart rate and blood flow is crucial for studying cardiovascular dysfunction, which leads to cardiovascular diseases.
Aim:
This study aims at in-depth understanding of high-speed cardiovascular dynamics in a zebrafish embryo model for various biomedical applications via frequency-comb-referenced quantitative phase imaging (FCR-QPI).
Approach:
Quantitative phase imaging (QPI) has emerged as a powerful technique in the field of biomedicine but has not been actively applied to the monitoring of circulatory/cardiovascular parameters, due to dynamic speckles and low frame rates. We demonstrate FCR-QPI to measure heart rate and blood flow in a zebrafish embryo. FCR-QPI utilizes a high-speed photodetector instead of a conventional camera, so it enables real-time monitoring of individual red blood cell (RBC) flow.
Results:
The average velocity of zebrafish's RBCs was measured from 192.5 to 608.8 μm / s at 24 to 28 hour-post-fertilization (hpf). In addition, the number of RBCs in a pulsatile blood flow was revealed to 16 cells/pulse at 48 hpf. The heart rates corresponded to 94 and 142 beats-per-minute at 24 and 48 hpf.
Conclusions:
This approach will newly enable in-depth understanding of the cardiovascular dynamics in the zebrafish model and possible usage for drug discovery applications in biomedicine.
