Effect of dissolved oxygen on N₂O release in the sewer system during controlling hydrogen sulfide by nitrate dosing

Nitrate dosing is commonly used for controlling hydrogen sulfide in sewer systems. However, it may potentially facilitate N₂O emission due to the denitrification process promoted by nitrate addition. In this study, lab-scale sewer reactors were operated to investigate the impact of nitrate addition on N₂O production in sewer systems. Results showed that the N₂O flux even increased by six times with the addition of nitrate when dissolved oxygen (DO) in the wastewater exceeded 0.4 mg/L. Principal component analysis showed that the N₂O concentration was notably affected by DO and oxidation-reduction potential (ORP) in the wastewater. Furthermore, it was founded that N₂O flux had a strong linear relationship with the DO concentration in the batch test. The microbial analysis found that the nosZ possessing organisms decreased significantly in the micro-aerobic condition and the copy numbers of nosZ gene declined consequently. It indicated that the inhibition of N₂O reduced to N₂ was responsible for significant accumulation and emission of N₂O in the micro-aerobic condition. Given the gravity sewers are not completely anaerobic, the DO concentration is ranged from 0.1 to 2.4 mg/L in gravity sewers with the partially filled flow. Therefore, more attention should be paid to the N₂O production when nitrate dosing for hydrogen sulfide controlling in gravity sewers.