Eukaryotic DNA replication with purified budding yeast proteins

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Updated: Oct 13, 2025

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
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Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

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Viktor Posse1, Erik Johansson2, John F X Diffley3

  • 1Chromosome Replication Laboratory, The Francis Crick Institute, London, United Kingdom; Department of Medical Biochemistry and Cell Biology, University of Gothenburg, Gothenburg, Sweden.

Methods in Enzymology
|November 15, 2021
PubMed

Keywords:
Chromosome duplicationDNA replicationDNA replication assayDNA replication in vitroProtein expressionProtein purificationReconstituted yeast DNA replication

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