NaF reduces KLK4 expression by decreasing Foxo1/Runx2 expression in LS8 cells

Area of Science:

• Biochemistry
• Molecular Biology
• Cell Biology

Background:

• High fluoride exposure is a significant environmental concern affecting various biological processes.
• Runt-related transcription factor 2 (Runx2) plays a crucial role in cellular differentiation and gene expression.
• The interplay between transcription factors and enamel-related genes is critical for ameloblast function.

Purpose of the Study:

• To investigate the impact of high fluoride on Runx2 expression in ameloblasts.
• To explore the relationship between Runx2, Foxo1, and KLK4 in fluoride-treated ameloblasts.

Main Methods:

• Ameloblast-like LS8 cells were treated with sodium fluoride (NaF).
• Gene silencing and overexpression techniques were used for Runx2 and Foxo1.
• mRNA and protein levels of Runx2, Foxo1, KLK4, and MMP20 were quantified using qRT-PCR and western blotting.

Main Results:

• Sodium fluoride (NaF) decreased Runx2 expression in a dose- and time-dependent manner.
• Runx2 knockdown reduced KLK4 expression under NaF conditions.
• Foxo1 modulated Runx2 expression; forced Foxo1 upregulated Runx2, while Foxo1 knockdown decreased it, an effect amplified by NaF.

Conclusions:

• High fluoride exposure downregulates Runx2 expression in ameloblasts.
• Fluoride-induced reduction in Foxo1/Runx2 expression contributes to decreased KLK4 expression.