Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reliable repurposing of the antibody interactome inside the cell.

Nature communications·2026
Same author

The Concise Guide to PHARMACOLOGY 2025/26: Ion channels.

British journal of pharmacology·2025
Same author

Kv2/Kv6.4 Heteromeric Potassium Channels Are Expressed in Spinal Motoneurons and Localized at C-Bouton Synapses.

The European journal of neuroscience·2025
Same author

clonevdjseq: A workflow and bioinformatics management system for sequencing, archiving, and analysis of VDJ sequences from clonal libraries.

BMC bioinformatics·2025
Same author

Kv2/Kv6.4 heteromeric potassium channels are expressed in spinal motor neurons and localized at C-bouton synapses.

bioRxiv : the preprint server for biology·2025
Same author

Open-source antibodies as a path to enhanced research reproducibility and transparency.

New biotechnology·2025

Related Experiment Video

Updated: Oct 13, 2025

Intraventricular Transplantation of Engineered Neuronal Precursors for In Vivo Neuroarchitecture Studies
15:00

Intraventricular Transplantation of Engineered Neuronal Precursors for In Vivo Neuroarchitecture Studies

Published on: May 11, 2019

5.8K

Genetically encoded intrabodies as high-precision tools to visualize and manipulate neuronal function.

James S Trimmer1

  • 1Department of Physiology and Membrane Biology, University of California Davis School of Medicine, 4303 Tupper Hall, Davis, CA 95616, USA..

Seminars in Cell & Developmental Biology
|November 16, 2021
PubMed
Summary

Intracellular antibodies, or intrabodies, are genetically encoded tools for neuroscience research. They precisely target molecules within neurons to study and manipulate brain function.

Keywords:
BrainFingRIntrabodyLive cell imagingNanobodyNeuronPlasmidRecombinantScFv

More Related Videos

Expanding the Toolkit for In Vivo Imaging of Axonal Transport
09:24

Expanding the Toolkit for In Vivo Imaging of Axonal Transport

Published on: December 23, 2021

3.6K
In Vivo Direct Reprogramming of Resident Glial Cells into Interneurons by Intracerebral Injection of Viral Vectors
12:26

In Vivo Direct Reprogramming of Resident Glial Cells into Interneurons by Intracerebral Injection of Viral Vectors

Published on: June 17, 2019

9.6K

Related Experiment Videos

Last Updated: Oct 13, 2025

Intraventricular Transplantation of Engineered Neuronal Precursors for In Vivo Neuroarchitecture Studies
15:00

Intraventricular Transplantation of Engineered Neuronal Precursors for In Vivo Neuroarchitecture Studies

Published on: May 11, 2019

5.8K
Expanding the Toolkit for In Vivo Imaging of Axonal Transport
09:24

Expanding the Toolkit for In Vivo Imaging of Axonal Transport

Published on: December 23, 2021

3.6K
In Vivo Direct Reprogramming of Resident Glial Cells into Interneurons by Intracerebral Injection of Viral Vectors
12:26

In Vivo Direct Reprogramming of Resident Glial Cells into Interneurons by Intracerebral Injection of Viral Vectors

Published on: June 17, 2019

9.6K

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • Antibodies are crucial reagents in neuroscience research.
  • Intracellular antibodies (intrabodies) are recombinant antibodies expressed within neurons.
  • Intrabodies offer precise targeting and manipulation of neuronal function.

Purpose of the Study:

  • To provide background on intrabody development and applications.
  • To highlight the utility of intrabodies in basic neuroscience research.
  • To showcase intrabodies as advanced research tools.

Main Methods:

  • Genetic encoding of recombinant antibodies for neuronal expression.
  • Targeting of specific molecules and subcellular domains within neurons.
  • Utilizing intrabodies to deliver cargo like fluorescent proteins or enzymes.

Main Results:

  • Intrabodies enable high-precision reporting and manipulation of neuronal function.
  • Their cell-type specific expression and selective binding are key advantages.
  • Intrabodies are emerging as valuable tools for complex neuroscience studies.

Conclusions:

  • Intrabodies represent a distinct subclass of antibodies with unique advantages for neuroscience.
  • Their application allows for detailed investigation of neuronal complexity.
  • Intrabodies are increasingly important for advancing basic neuroscience research.