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Related Concept Videos

X-Inactivation01:58

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Functional Cloning Using a Xenopus Oocyte Expression System
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FOXC1.

Ming Li1,2,3, Hong Lv1,2,3, Siyuan Zhong1,2,3

  • 1From the Department of Pathology, Fudan University Shanghai Cancer Center.

Archives of Pathology & Laboratory Medicine
|November 16, 2021
PubMed
Summary
This summary is machine-generated.

Forkhead box C1 (FOXC1) protein is a highly specific marker for triple-negative breast cancer (TNBC). Immunohistochemistry detection of FOXC1 aids in TNBC subclassification and diagnosis.

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Area of Science:

  • Oncology
  • Molecular Biology
  • Pathology

Background:

  • Limited research exists on FOXC1 protein expression across invasive breast cancer subtypes.
  • Immunohistochemistry (IHC)-based surrogate molecular classification is crucial for breast cancer subtyping.

Purpose of the Study:

  • To assess the diagnostic utility of the IHC-based FOXC1 test for breast cancer subtyping.
  • To evaluate the correlation between FOXC1 expression and clinicopathologic parameters in triple-negative breast cancer (TNBC).

Main Methods:

  • FOXC1 expression was analyzed using IHC in a cohort of 2443 breast cancer patients.
  • Receiver operating characteristic (ROC) curves were employed to determine FOXC1's diagnostic accuracy for the triple-negative phenotype.
  • FOXC1 expression was correlated with TNBC clinicopathologic parameters.

Main Results:

  • FOXC1 expression was significantly higher in TNBC compared to other subtypes.
  • FOXC1 demonstrated high diagnostic value in predicting the triple-negative phenotype, with a 1% cutoff maximizing sensitivity and specificity.
  • In TNBC, FOXC1 expression correlated with aggressive tumor phenotypes and basal markers, but not with apocrine markers.

Conclusions:

  • FOXC1 serves as a highly specific marker for identifying the triple-negative breast cancer phenotype.
  • IHC detection of FOXC1 is a valuable tool for the diagnosis and subclassification of TNBC.