Search research articles

Related Concept Videos

Cis-regulatory Sequences

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Fundamental and unique roles of PLAC1 in the regulation of rat and human trophoblast cell development.

Development (Cambridge, England)·2026

Same author

Characterization of a cis-regulatory element upstream of matrix metalloproteinase-9.

Scientific reports·2025

Same author

PPARG directs trophoblast cell fate and establishment of the uterine-placental interface.

bioRxiv : the preprint server for biology·2025

Same author

Two classes of syncytiotrophoblast nuclei form following BAP-driven human pluripotent stem cell differentiation.

Placenta·2025

Same author

DNA origami drives gene expression in a human cell culture system.

Scientific reports·2024

Same author

Conditionally mutant animal model for investigating the invasive trophoblast cell lineage.

Development (Cambridge, England)·2023

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Oct 13, 2025

Mouse In Vivo Placental Targeted CRISPR Manipulation

Mouse In Vivo Placental Targeted CRISPR Manipulation

Published on: April 14, 2023

Mapping cis-regulatory elements in the midgestation mouse placenta.

Rebekah R Starks^1,2, Haninder Kaur¹, Geetu Tuteja^3,4

¹Genetics, Development, and Cell Biology, Iowa State University, Ames, IA, 50011, USA.

Scientific Reports

|November 17, 2021

Summary

This summary is machine-generated.

Investigating gene regulation in the midgestation mouse placenta, this study mapped active and poised regulatory elements. Findings reveal distinct roles for promoters and enhancers in placental development and identify novel transcription factors for future research.

More Related Videos

Dissection and Explant Culture of Murine Allantois for the In Vitro Analysis of Allantoic Attachment

Dissection and Explant Culture of Murine Allantois for the In Vitro Analysis of Allantoic Attachment

Published on: January 13, 2018

Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program

Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program

Published on: December 16, 2016

Related Experiment Videos

Last Updated: Oct 13, 2025

Mouse In Vivo Placental Targeted CRISPR Manipulation

Mouse In Vivo Placental Targeted CRISPR Manipulation

Published on: April 14, 2023

Dissection and Explant Culture of Murine Allantois for the In Vitro Analysis of Allantoic Attachment

Dissection and Explant Culture of Murine Allantois for the In Vitro Analysis of Allantoic Attachment

Published on: January 13, 2018

Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program

Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program

Published on: December 16, 2016

Area of Science:

Developmental Biology
Genomics
Epigenetics

Background:

Placental defects can cause severe pregnancy complications.
Gene misregulation is a key cause of placental defects.
Regulatory mechanisms in the midgestation placenta are poorly understood.

Purpose of the Study:

To investigate gene regulatory mechanisms in the midgestation mouse placenta.
To generate and analyze ChIP-seq data for histone modifications.
To annotate cis-regulatory regions and identify transcription factors.

Main Methods:

ChIP-sequencing (ChIP-seq) for histone modifications.
Annotation of active/poised promoters and enhancers.
Motif analysis of cis-regulatory regions.

Main Results:

Poised promoters linked to neuronal development; active promoters to housekeeping genes.
Active and poised enhancers associated with placental development.
Active enhancers linked to placenta-specific genes; identified novel transcription factors.

Conclusions:

The study provides a foundational dataset for placental gene regulation research.
Identified key regulatory elements and transcription factors involved in placental development.
Highlights the importance of enhancers in placenta-specific gene expression.