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Published on: December 3, 2015
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DNA origami drives gene expression in a human cell culture system
Chang Yong Oh1,2, Haninder Kaur3, Geetu Tuteja3
1Department of Biochemistry and Molecular Biology, Iowa State University, Ames, IA, 50011, USA. dngk3325@gmail.com.
Scientific Reports
|November 9, 2024
Summary
DNA origami nanoparticles can be expressed in human cells. Modifying single-stranded DNA domains and genetic elements within these DNA nanoparticles can regulate gene expression, advancing nanomedicine.
Area of Science:
- Biotechnology
- Nanotechnology
- Molecular Biology
Background:
- Self-assembling DNA nanoparticles offer flexible platforms for molecular cargo delivery.
- DNA origami nanoparticles can be transcribed and translated in vitro and in cells.
- Previous work demonstrated gene editing and expression using DNA nanoparticles.
Purpose of the Study:
- Investigate gene expression from DNA origami nanoparticles in a human cell line.
- Determine the impact of single-stranded DNA (ssDNA) domains on gene expression.
- Analyze the role of promoter and enhancer sequence arrangement in gene expression.
Main Methods:
- Utilized DNA origami nanoparticles containing genetic elements in a human cell line.
- Varied the presence, density, and location of ssDNA domains.
- Assessed gene expression levels based on nanoparticle architecture.
Main Results:
- Human cells successfully express genes within highly folded DNA origami architectures.
- The presence, density, and location of ssDNA domains significantly influence gene expression levels.
- Architectural arrangement of promoter and enhancer sequences affects gene expression outcomes.
Conclusions:
- DNA origami nanoparticle architecture can be tuned to regulate gene expression.
- ssDNA domains are critical factors in controlling gene expression within these nanostructures.
- These findings support the potential of DNA nanoparticles for biotherapeutics like gene editing and vaccines.
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