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Updated: Oct 13, 2025
Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
Published on: April 14, 2015
A self-quenching fluorescence probe-mediated exponential isothermal amplification system for highly sensitive and
Jun Zhao1, Jiandong Wu2, Xiaosong Wu1,3
1Center of Engineering Technology Research for Biomedical Optical Instrument, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, P. R. China. zhul@aiofm.ac.cn.
Abstract:
We designed an efficient self-quenching fluorescence probe and constructed this probe-mediated exponential isothermal amplification system for miRNA detection. Owing to the significant improvement in the detective signal-to-background ratio, a wide dynamic range of 9 orders of magnitude and a limit of detection as low as 0.08 aM can be easily achieved in a single step. Furthermore, benefiting from the additional advantages of high specificity and biocompatibility, the proposed method has been demonstrated to be capable of accurately quantifying miRNA biomarkers in serum, which will provide promising perspectives for clinical diagnosis.
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