Related Experiment Video
Updated: Oct 13, 2025
03:08
Using Human Differentially Expressed Gene Lists to Perform Downstream Pathway Enrichment Analysis and Target Prioritization
Published on: October 3, 2025
71
The reactome pathway knowledgebase 2022
Marc Gillespie1,2, Bijay Jassal1, Ralf Stephan1
1Ontario Institute for Cancer Research, Toronto, ON M5G0A3, Canada.
Nucleic Acids Research
|November 17, 2021
Summary
The Reactome Knowledgebase offers detailed human biological pathways for research. It aids in understanding diseases and analyzing complex biological data, including host responses to infections.
Area of Science:
- Molecular Biology
- Systems Biology
- Bioinformatics
Background:
- The Reactome Knowledgebase is a curated resource detailing human biological processes.
- It models molecular transformations in a consistent data structure.
- This resource aids in understanding both normal physiology and disease pathology.
Purpose of the Study:
- To present recent updates and expanded annotations within the Reactome Knowledgebase.
- To highlight new tools for analyzing high-throughput biological data.
- To improve the contextualization of understudied proteins within biological pathways.
Main Methods:
- Manual curation of molecular details for human biological processes.
- Annotation of signaling pathways, including those related to infections (SARS-CoV-1, SARS-CoV-2).
- Development of new analytical tools for multi-source high-throughput data integration.
Main Results:
- Expanded annotations for normal and disease-associated signaling pathways.
- Detailed information on drugs targeting these pathways.
- Enhanced capabilities for analyzing gene expression and mutation data.
- Improved methods for integrating and contextualizing data from understudied proteins.
Conclusions:
- Reactome serves as a vital digital archive and analytical tool for biological research.
- Recent updates enhance the study of infectious diseases and host responses.
- New tools facilitate deeper insights from complex, multi-source biological datasets.
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