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Assessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling
Published on: October 28, 2014
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RNA-binding proteins and translation control in angiogenesis
Madeleine R Smith1, Guilherme Costa1
1Wellcome-Wolfson Institute for Experimental Medicine, Queen's University, Belfast, UK.
The FEBS Journal
|November 19, 2021
Summary
RNA-binding proteins (RBPs) and translation control are crucial for new blood vessel formation (angiogenesis). Dysregulation of these processes, particularly RBP-mRNA interactions, contributes to diseases and offers therapeutic targets.
Area of Science:
- Molecular Biology
- Cell Biology
- Biochemistry
Background:
- Angiogenesis, the formation of new blood vessels, is vital for development and regeneration, requiring complex cellular signaling.
- RNA-binding proteins (RBPs) and protein translation are key regulators of cellular responses to environmental cues.
- Specific RBPs, including HuR, TIS11, hnRNPs, NF90, QKIs, and YB1, are implicated in both normal and abnormal angiogenesis.
Purpose of the Study:
- To review the critical roles of RBP-mediated RNA regulation in angiogenesis.
- To explore the impact of translation control mechanisms on new blood vessel generation.
- To highlight therapeutic strategies targeting post-transcriptional gene expression in angiogenesis.
Main Methods:
- Literature review of existing research on RBPs, mRNA metabolism, and protein synthesis in angiogenesis.
- Analysis of studies demonstrating the involvement of specific RBPs in physiological and pathological angiogenesis.
- Examination of evidence linking altered RBP-mRNA interactions and translation to disease states.
Main Results:
- RBPs critically regulate the metabolism of mRNAs encoding angiogenic factors.
- RBP-mRNA interactions are frequently altered in disease conditions associated with aberrant angiogenesis.
- Protein synthesis mechanisms significantly influence the generation of new blood vessels.
Conclusions:
- RBP activity and translation control are fundamental post-transcriptional mechanisms governing angiogenesis.
- Dysregulation of these processes contributes to pathological angiogenesis.
- Targeting RBP-mediated RNA regulation and translation offers promising therapeutic avenues for controlling unwanted blood vessel formation.
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