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A compound post-column re-focusing approach in supercritical fluid chromatography
Mingzhe Sun1, Peter Schoenmakers1
1Analytical Chemistry Group, Van't Hoff Institute for Molecular Sciences, University of Amsterdam, 1098 XH Amsterdam, The Netherlands; Centre for Analytical Sciences Amsterdam (CASA), The Netherlands.
Journal of Chromatography. A
|November 22, 2021
Summary
This study introduces a post-column re-focusing technique for supercritical-fluid chromatography (SFC) to improve detection sensitivity. The method enhances analyte signals and concentrations, overcoming limitations of CO2-based mobile phases.
Area of Science:
- Analytical Chemistry
- Chromatography
Background:
- Supercritical-fluid chromatography (SFC) is gaining prominence due to instrumentation advancements.
- CO2-based mobile phases and high flow rates in SFC pose challenges for trace analysis and detector coupling.
Purpose of the Study:
- To develop a post-column re-focusing approach for SFC analysis.
- To achieve signal and concentration enhancement for UV-Vis detection in SFC.
Main Methods:
- A post-column re-focusing strategy involving heart-cutting fractions into a trapping column.
- Analyte re-mobilization using a flushing solvent after CO2 elimination.
- Optimization of trapping stationary phase and solvents for selected compounds.
Main Results:
- Achieved signal enhancement ratios between 2.2 and 6.4 for four representative compounds.
- Observed actual concentration enhancement ratios between 1.7 and 2.9.
- Demonstrated the effectiveness of the method across varying SFC modifier percentages.
Conclusions:
- The proposed post-column re-focusing technique effectively enhances analyte detection in SFC.
- This method addresses sensitivity limitations associated with CO2-based SFC.
- The approach offers a viable solution for trace analysis in SFC applications.
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