Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

9.6K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
9.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

High dynamic range structured illumination microscopy based on multi-channel fusion and dual-phase binary fringe.

Optics express·2026
Same author

The impact of clinical internship experiences on professional identity among nursing undergraduates: A qualitative study based on control-value theory.

Nurse education today·2026
Same author

High-speed multimodal intravascular ultrasound and photoacoustic imaging system for atherosclerosis characterization.

Biomedical optics express·2026
Same author

EPI-guided adaptive refocusing for high-SNR 3D measurement in a large-DOF MEMS-based structured light field.

Optics express·2026
Same author

Polarization-based structured light 3D measurement for metallic surfaces under time-varying ambient illumination.

Optics express·2026
Same author

Machine-learning CT radiomics for prognostication in unresectable pancreatic cancer.

Frontiers in pharmacology·2026
Same journal

Denoising algorithm of Φ-OTDR systems based on adaptive fractional wavelet transform denoising.

Optics express·2026
Same journal

Millisecond photon-to-photon latency and high-speed volumetric projection system for optogenetics.

Optics express·2026
Same journal

Polarization-encoded coaxial structured light for high-precision 3D surface profilometry.

Optics express·2026
Same journal

Discrete freeform optical design based on collaborative optimization of point cloud and local normals.

Optics express·2026
Same journal

Ultrafast ghost imaging with 25 GHz speckle switching and wavelength-division multiplexing.

Optics express·2026
Same journal

Atomic vapor cells fabricated by femtosecond laser welding of standard-optical-quality glass.

Optics express·2026
See all related articles

Related Experiment Video

Updated: Oct 12, 2025

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

9.1K

New strategy for high-dimensional single-pixel imaging.

Xianye Li, Yongkai Yin, Wenqi He

    Optics Express
    |November 23, 2021
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces a novel single-pixel imaging (SPI) strategy for high-dimensional imaging, enabling low-cost 3D reconstruction, color texture acquisition, and viewpoint expansion. The technique utilizes multiple detectors and advanced fusion methods for accurate data capture.

    More Related Videos

    Single Molecule Fluorescence Microscopy on Planar Supported Bilayers
    20:00

    Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

    Published on: October 31, 2015

    14.1K
    Lensless Fluorescent Microscopy on a Chip
    11:23

    Lensless Fluorescent Microscopy on a Chip

    Published on: August 17, 2011

    17.8K

    Related Experiment Videos

    Last Updated: Oct 12, 2025

    Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
    12:51

    Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

    Published on: December 9, 2013

    9.1K
    Single Molecule Fluorescence Microscopy on Planar Supported Bilayers
    20:00

    Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

    Published on: October 31, 2015

    14.1K
    Lensless Fluorescent Microscopy on a Chip
    11:23

    Lensless Fluorescent Microscopy on a Chip

    Published on: August 17, 2011

    17.8K

    Area of Science:

    • Optics and Photonics
    • Computational Imaging
    • Computer Vision

    Background:

    • Single-pixel imaging (SPI) offers a cost-effective alternative to traditional imaging systems by minimizing detector resolution requirements.
    • Existing SPI techniques are primarily limited to 2D imaging or basic 3D reconstruction, lacking comprehensive high-dimensional data acquisition capabilities.
    • There is a need for advanced SPI methods that can simultaneously capture depth, color, and texture information with improved accuracy and reduced computational complexity.

    Purpose of the Study:

    • To propose and demonstrate a comprehensive single-pixel imaging strategy for high-dimensional data acquisition, including 3D shape, color texture, and viewpoint expansion.
    • To develop a low-cost 3D imaging system using SPI, incorporating structured illumination and grating-based height encoding.
    • To enhance the accuracy of 3D shape fusion and color fidelity through novel data processing techniques.

    Main Methods:

    • A low-cost single-pixel 3D imaging system employing a raster scanner for structured illumination and a grating for height encoding.
    • Utilization of two single-pixel detectors (SPDs) from different angles to capture height information, with a modified total variation criterion for error reduction in shape fusion.
    • Integration of a third SPD with bandpass filters for color texture acquisition and a viewpoint switching method inspired by shape-from-shading for improved color fidelity.

    Main Results:

    • Successful implementation of a single-pixel 3D imaging scheme with reduced blocking area and enhanced shape fusion accuracy.
    • Acquisition of color texture information using multiple SPDs and bandpass filters, demonstrating capability for higher dimensional data capture.
    • Improved color fidelity through a novel viewpoint switching method, showcasing the potential for comprehensive high-dimensional imaging with SPI.

    Conclusions:

    • The proposed SPI strategy represents a significant advancement in high-dimensional imaging, offering a versatile and cost-effective solution.
    • This work provides a foundational demonstration for the application of SPI in the acquisition, reconstruction, and fusion of complex, high-dimensional image data.
    • The developed technique has broad implications for various fields requiring detailed 3D and multi-spectral imaging with minimal hardware complexity.