Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

8.6K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
8.6K
Cryo-electron Microscopy01:28

Cryo-electron Microscopy

3.7K
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
3.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Incremental Effectiveness of a Second Varicella Vaccine in Children: A Prospective Cohort Study in Anhui, China.

Vaccines·2026
Same author

Exploring nanographene for single molecule imaging at cryogenic temperatures.

The Journal of chemical physics·2026
Same author

Application of gradient-consensus Richardson-Lucy deconvolution to noisy undersampled brightfield microscopy data.

Biomedical optics express·2026
Same author

Zebrafish Ace2N-mNeon expression toolkit for <i>in vivo</i> voltage imaging of neuronal synchrony and cardiac maturation.

Neurophotonics·2026
Same author

Absorption dipole effects on MINFLUX single molecule localization.

Optics express·2026
Same author

Plasmonic Enhancement of Fluorescence and Protein Dynamics in Living Mammalian Cells.

Advanced materials (Deerfield Beach, Fla.)·2026
Same journal

Long-term stabilization of intensity-difference squeezing from four-wave mixing in rubidium vapor.

Optics express·2026
Same journal

Robust 3D topography measurement of large-range high-aspect-ratio structures based on dual-domain statistical filtering in SD-OCT.

Optics express·2026
Same journal

Broadband transmissive terahertz metasurface for simultaneous quad-mode OAM multiplexing.

Optics express·2026
Same journal

Leveraging two-dimensional materials for high-sensitivity optical sensors: quasi-bound states in the continuum within hybrid metasurfaces.

Optics express·2026
Same journal

Resolution investigation for dual-spherical-wave optical scanning holographic microscopy: methods and performance.

Optics express·2026
Same journal

Robustness of parallel subnetwork-filtered diffractive deep neural networks.

Optics express·2026
See all related articles

Related Experiment Video

Updated: Oct 12, 2025

Visualization of Cortex Organization and Dynamics in Microorganisms, using Total Internal Reflection Fluorescence Microscopy
14:14

Visualization of Cortex Organization and Dynamics in Microorganisms, using Total Internal Reflection Fluorescence Microscopy

Published on: May 1, 2012

11.7K

Waveguide-based total internal reflection fluorescence microscope enabling cellular imaging under cryogenic

Qingru Li, Christiaan N Hulleman, Robert J Moerland

    Optics Express
    |November 23, 2021
    PubMed
    Summary
    This summary is machine-generated.

    We developed a new waveguide-based total internal reflection fluorescence (TIRF) microscope for cryogenic imaging. This method enables stable, high-resolution cellular observation at low temperatures, overcoming limitations of traditional TIRF systems.

    More Related Videos

    High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
    14:09

    High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

    Published on: November 16, 2019

    7.1K
    Oligomerization Dynamics of Cell Surface Receptors in Living Cells by Total Internal Reflection Fluorescence Microscopy Combined with Number and Brightness Analysis
    10:43

    Oligomerization Dynamics of Cell Surface Receptors in Living Cells by Total Internal Reflection Fluorescence Microscopy Combined with Number and Brightness Analysis

    Published on: November 6, 2019

    6.9K

    Related Experiment Videos

    Last Updated: Oct 12, 2025

    Visualization of Cortex Organization and Dynamics in Microorganisms, using Total Internal Reflection Fluorescence Microscopy
    14:14

    Visualization of Cortex Organization and Dynamics in Microorganisms, using Total Internal Reflection Fluorescence Microscopy

    Published on: May 1, 2012

    11.7K
    High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
    14:09

    High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

    Published on: November 16, 2019

    7.1K
    Oligomerization Dynamics of Cell Surface Receptors in Living Cells by Total Internal Reflection Fluorescence Microscopy Combined with Number and Brightness Analysis
    10:43

    Oligomerization Dynamics of Cell Surface Receptors in Living Cells by Total Internal Reflection Fluorescence Microscopy Combined with Number and Brightness Analysis

    Published on: November 6, 2019

    6.9K

    Area of Science:

    • Biophysics
    • Cell Biology
    • Microscopy

    Background:

    • Total internal reflection fluorescence (TIRF) microscopy is vital for studying cellular events near the plasma membrane.
    • Cryogenic imaging preserves near-native states and enhances fluorophore photostability by reducing photo-bleaching.
    • Conventional TIRF microscopy has limitations including small fields of view and incompatibility with cryogenic conditions.

    Purpose of the Study:

    • To develop a novel TIRF microscope capable of operating under cryogenic conditions.
    • To overcome the field-of-view limitations and cryogenic incompatibility of traditional TIRF systems.
    • To provide an accessible method for high-resolution cellular imaging at low temperatures.

    Main Methods:

    • Utilized high refractive index waveguides on photonic chips to generate a uniform evanescent field.
    • Integrated waveguide technology with TIRF microscopy principles.
    • Enabled cellular observation at cryogenic temperatures.

    Main Results:

    • Successfully generated a uniform evanescent field using waveguide technology.
    • Achieved cellular observation under cryogenic conditions using the novel TIRF microscope.
    • Demonstrated an inexpensive approach for cryogenic TIRF imaging.

    Conclusions:

    • The waveguide-based TIRF microscope offers a cost-effective solution for cryogenic cellular imaging.
    • This technology expands the capabilities of TIRF microscopy for studying biological structures at low temperatures.
    • The system provides enhanced photostability and preserves cellular integrity for advanced research.