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Updated: Oct 12, 2025

Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues
Published on: June 21, 2018
Improved coverage of plant metabolites using powder laser desorption/ionization coupled with Fourier-transform ion
Syful Islam1, Rafiqul Alam2, Sunghwan Kim3
1Department of Chemistry, Kyungpook National University, 80 Daehak-ro, Buk-gu, Daegu 41566, Republic of Korea; Department of Environment, Munshiganj District Office, Munshiganj 1500, Bangladesh.
Abstract:
In this study, the effectiveness of powder laser desorption/ionization (LDI) coupled with Fourier-transform ion cyclotron mass spectrometry (FT-ICR MS) was evaluated for the improved coverage of plant metabolites. Ground plant (powder) was fixed on a metal plate using double-sided tape. Compared with the conventional approach involving liquid extraction, which is followed by electrospray ionization MS analysis, a smaller amount of sample (∼200 µg) was required in the proposed method. Additionally, the laborious steps of liquid extraction, concentration, and solid-phase extraction were avoided. Employing the proposed method in Centella asiatica leaves analysis, higher number of reproducible molecular formulas (>5000) and metabolites (>650) were obtained than the conventional methods. Flavonoids, phenolic acids, xanthones, lipids, carbohydrates, terpenoids, and alkaloids compounds were detected from leaves, stems, and roots of C. asiatica. This study indicates that LDI FT-ICR MS is a quick and effective tool for enhanced plant metabolite profiling in the solid phase.
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