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Prostaglandin endoperoxides 21. Covalent binding of levuglandin E2 with proteins
R G Salomon1, M R Jirousek, S Ghosh
1Department of Chemistry, Case Western Reserve University, Cleveland, Ohio 44106-2699.
Prostaglandins
|November 1, 1987
Abstract:
Levuglandin E2 (LGE2), a gamma-ketoaldehyde produced by rearrangement of the prostaglandin endoperoxide PGH2 under the aqueous conditions of its biosynthesis, binds covalently with ram seminal vesicle microsomes. Totally synthetic 5,6-ditritio-LGE2 was prepared and used to determine that rapid covalent binding of LGE2 (initially 800 microM) occurs with 6.4 microM bovine serum albumin (greater than 10 equiv within 1 min) which approaches saturation (approximately 16 equiv) after 40 min at 37 degrees C.