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A comparison study between ragweed-specific IgG4 as measured by RAST and FAST
F A Rommel1, H Lee, N F Adkinson
1Department of Medicine, Johns Hopkins School of Medicine, Baltimore, MD 21239.
Abstract:
A solid phase Sepharose-based RIA (IgG4 RAST) was compared to the IgG4 FAST enzyme immunoassay for measurements of Ragweed-specific IgG4. Although FAST reagents were used, a standard serum of known antibody content along with modifications in the FAST assay were necessary to attempt to develop a quantitative assay. Various assay parameters such as precision, reproducibility, sensitivity and parallelism were examined. Specificity of the monoclonal anti-IgG4 used in the FAST was not evaluated. Conditions and method of assay for the two systems differed substantially, particularly in the short incubation times for the IgG4 determinations.
