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Mammalian Cell Culture as a Platform for Veterinary Vaccines
Thailín Lao González1, Ileanet Ávalos Olivera1, Alina Rodríguez-Mallon2
1Animal Biotechnology Department, Center for Genetic Engineering and Biotechnology, Havana, Cuba.
Methods in Molecular Biology (Clifton, N.J.)
|November 24, 2021
Summary
Mammalian cell culture, once costly, is now cost-effective for producing therapeutic proteins. Innovations in suspension cultures and defined media have made this technology accessible for veterinary vaccines and other applications.
Area of Science:
- Biotechnology
- Cell Biology
- Biopharmaceutical Manufacturing
Background:
- Mammalian cells have been a primary host for producing therapeutic recombinant proteins for over 30 years.
- High costs associated with media and supplies initially limited their use to high-value human pharmaceuticals like antibodies.
Purpose of the Study:
- To detail the protocol for generating recombinant mammalian cell lines.
- To highlight advancements making mammalian cell culture more economically feasible.
Main Methods:
- Development of suspension culture techniques for individual cells.
- Implementation of chemically defined media lacking macromolecules.
Main Results:
- Significant reduction in the cost of mammalian cell culture.
- Expanded application of mammalian cell expression systems for veterinary protein production and vaccines.
Conclusions:
- Innovations have enhanced the technical and economic viability of mammalian cell-based protein production.
- Mammalian cell culture is now a practical platform for producing active pharmaceutical ingredients for veterinary applications.
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