Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Capillary Electrophoresis: Instrumentation

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...

Electrophoresis: Overview

Electrophoresis: Overview

Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...

Capillary Electrophoresis: Applications

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

Column Efficiency: Rate Theory

Column Efficiency: Rate Theory

The rate theory of chromatography provides quantitative insight into the shapes and widths of elution bands. These bands are based on the random-walk mechanism governing molecular migration within a column. The Gaussian profile of chromatographic bands arises from the cumulative effect of random molecular motions as they progress through the column.
During elution, a solute molecule experiences numerous transitions between stationary and mobile phases, exhibiting irregular residence times in...

High-Performance Liquid Chromatography: Elution Process

High-Performance Liquid Chromatography: Elution Process

In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...

Analyte Adsorption and Distribution

Analyte Adsorption and Distribution

In certain chromatographic separations, solutes transfer between the mobile phase and the stationary phase via sorption, which typically refers to the process of adsorption. For many chromatographic systems, the sorption process often depends on the polarity of the compounds—an expression of the overall dipole moment within the molecule. During the separation process, there is competition between the solute and solvent for adsorption to the stationary phase. Highly polar compounds and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Development of analytical functions with high-performance liquid phase separation systems on the basis of the moment theory.

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry·2025

Same author

Moment analysis method for the determination of permeation kinetics of coumarin at lipid bilayers of liposomes by using capillary electrophoresis.

Electrophoresis·2024

Same author

Moment Analysis Method for Measurement of Reaction Equilibrium and Rate Constants by Using High-Performance Liquid Chromatography.

Analytical chemistry·2024

Same author

Kinetic study on solute permeation at the interface of molecular aggregates by partial filling capillary electrophoresis.

Electrophoresis·2021

Same author

Moment Theory of Chromatography for the Analysis of Reaction Kinetics of Intermolecular Interactions.

Analytical chemistry·2021

Same author

Moment theory for the analytical determination of rate constants for solute permeation at the interface of spherical molecular aggregates.

Electrophoresis·2019

Search research articles

Related Experiment Video

Updated: Oct 12, 2025

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Published on: November 21, 2023

Moment equations for partial filling capillary electrophoresis.

¹Department of Chemistry, Faculty of Science, Rikkyo University, Tokyo, Japan.

Electrophoresis

|November 24, 2021

Summary

Moment equations were developed for partial filling capillary electrophoresis (CE) systems to analyze solute dissolution and intermolecular interactions. These equations provide a theoretical basis for studying solute permeation and reaction kinetics involving molecular assemblies.

Keywords:

Interfacial solute permeation Intermolecular interaction Moment analysis theory Partial filling capillary electrophoresis Spherical molecular assembly

More Related Videos

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

Published on: October 24, 2018

Absolute Quantitation of Inositol Pyrophosphates by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry

Absolute Quantitation of Inositol Pyrophosphates by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry

Published on: August 13, 2021

Related Experiment Videos

Last Updated: Oct 12, 2025

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System

Published on: November 21, 2023

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry

Published on: October 24, 2018

Absolute Quantitation of Inositol Pyrophosphates by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry

Absolute Quantitation of Inositol Pyrophosphates by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry

Published on: August 13, 2021

Area of Science:

Analytical Chemistry
Physical Chemistry

Background:

Partial filling capillary electrophoresis (CE) is utilized to study solute dissolution and intermolecular interactions.
Understanding solute migration and assembly behavior is crucial for analyzing these phenomena.

Purpose of the Study:

To develop moment equations for partial filling CE systems, accounting for solute dissolution and intermolecular interactions.
To provide a theoretical framework for analyzing complex CE behaviors under various experimental conditions.

Main Methods:

Development of moment equations based on the Einstein equation for diffusion and the random walk model.
Systematic derivation of equations for five distinct experimental conditions based on relative migration velocities.
Application of derived equations to simulate partial filling CE behavior for solute dissolution into spherical molecular assemblies.

Main Results:

Moment equations were successfully developed for partial filling CE systems.
Simulations demonstrated the effectiveness of the moment equations in analyzing solute dissolution phenomena.
The study focused on cases where solute migration velocity exceeds that of molecular assemblies.

Conclusions:

The developed moment equations serve as a theoretical foundation for utilizing partial filling CE.
These equations enable the study of solute permeation kinetics at molecular assembly interfaces.
The research facilitates the investigation of reaction kinetics for intermolecular interactions.